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人々 2B4 / SLAMF / CD244 蛋白質 (Fc タグ)

  • Human 2B4 / SLAMF / CD244 Protein (Fc Tag) SDS-PAGE
データシートレビュー関連製品プロトコル
人々 2B4/SLAMF4 タンパク質製品情報
同意語:2B4,NAIL,NKR2B4,Nmrk,SLAMF4
タンパク質構造:A DNA sequence encoding the extracellular domain (Met 1-Arg221) of human 2B4 / CD244 (NP_057466.1) precursor was expressed with the C-terminal fused Fc region of human IgG1.
:Human
発現宿主:Human Cells
フォーム & 配送:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
人々 2B4/SLAMF4 タンパク質 QC 試験
純度:> 97 % as determined by SDS-PAGE
生物活性:1. Measured by its binding ability in a functional ELISA. Immobilized human CD48 at 10 μg/ml (100 μl/well) can bind recombinant human 2B4 / CD244 with a linear range of 0.004-0.4 μg/ml.
2. Measured by its ability to bind biotinylated mouse CD48 in a functional ELISA.
エンドトキシン:< 1.0 EU per μg of the protein as determined by the LAL method
安定性:Samples are stable for up to twelve months from date of receipt at -70℃
予測される N ターミナル:Cys 22
分子質量:The recombinant 2B4/CD244/Fc chimera is a disulfide-linked homodimer. The reduced monomer comprises 438 amino acids and predicts a molecular mass of 49 kDa. As a result of glycosylation, the rh2B4/CD244/Fc monomer migrates as an approximately 70-80 kDa band in SDS-PAGE under reducing conditions.
製剤:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
人々 2B4/SLAMF4 タンパク質使用ガイド
保存:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
人々 2B4/SLAMF4 Protein SDS-PAGE
Human 2B4 / SLAMF / CD244 Protein (Fc Tag) SDS-PAGE

2B4/SLAMF4 関連経路

    Other 2B4/SLAMF4 Recombinant Protein Products
    10042-H02H
    2B4 / CD244 背景

    The CD244 antigen, also known as 2B4, is a cell surface glycoprotein implicated in the regulation of natural killer and T lymphocyte function. 2B4 is a member of the signaling lymphocyte activation molecule (SLAM)-related receptor family and is important for stimulating NK cell cytotoxicity and cytokine production, which is expressed on all NK cells, a subpopulation of T cells, monocytes and basophils. The 2B4 antigen identified on NK cells and T cells is capable of transmitting stimulatory signals and non-MHC-restricted killing. Reported as an activating receptor, human 2B4 can effectively activate and enhance NK cell–mediated cytotoxicity, induce secretion of IFN-γ and matrix metalloproteinases (MMPs), as well as NK cell invasiveness. As a cell surface glycoprotein of the Ig-superfamily structurally related to CD2-like molecules such as CD2, CD48, CD58, CD84, Ly-9, and SLAM, 2B4 (CD244) is expressed on all human NK cells, a subpopulation of T cells, basophils and monocytes. 2B4 activates NK cell mediated cytotoxicity, induces secretion of IFN-gamma and matrix metalloproteinases, and NK cell invasiveness.

    Immune Checkpoint
    Immune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: IP Antibodies   Immune Checkpoint Detection: FCM Antibodies
    Immune Checkpoint Proteins
    Immune Checkpoint Targets   Co-inhibitory Immune Checkpoint Targets

    Immunotherapy   Cancer Immunotherapy   Targeted Therapy

    人々 2B4 / CD244 参考文献
  • Nakajima H, et al. (2000) 2B4: an NK cell activating receptor with unique specificity and signal transduction mechanism. Hum Immunol. 61(1): 39-43.
  • Chuang SS, et al. (2001) 2B4 (CD244)-mediated activation of cytotoxicity and IFN-gamma release in human NK cells involves distinct pathways. J Immunol. 167(11): 6210-6.
  • McNerney ME, et al. (2005) 2B4 (CD244) is a non-MHC binding receptor with multiple functions on natural killer cells and CD8+ T cells. Mol Immunol. 42(4): 489-94.
  • Mathew SO, et al. (2009) Functional role of human NK cell receptor 2B4 (CD244) isoforms. Eur J Immunol. 39(6): 1632-41.
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    Size / Price
    カタログ: 10042-H02H-50
    定価: 
    価格:      (You Save: )

    Datasheet & Documentation

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    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
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