Anti-2B4 / CD244 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-2B4 / CD244 Antibody
Validated applications
ELISA
Species reactivity
Reacts with: Human
Specificity
Human 2B4 / CD244
Immunogen
Recombinant Human 2B4 protein (Catalog#10042-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human 2B4 (rh 2B4; Catalog#10042-H08H; NP_057466.1; Met 1-Arg 221). Total IgG was purified by Protein A affinity chromatography
.
Source
Polyclonal Rabbit IgG
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-2B4 / CD244 Antibody (Rabbit Polyclonal antibody) Validated Applications
| Application |
Dilution |
Notes |
| ELISA |
1:1000-1:2000 |
|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-2B4 / CD244 Antibody Alternative Names
Anti-2B4 Antibody;Anti-NAIL Antibody;Anti-NKR2B4 Antibody;Anti-Nmrk Antibody;Anti-SLAMF4 Antibody
2B4 / CD244 Background Information
The CD244 antigen, also known as 2B4, is a cell surface glycoprotein implicated in the regulation of natural killer and T lymphocyte function. 2B4 is a member of the signaling lymphocyte activation molecule (SLAM)-related receptor family and is important for stimulating NK cell cytotoxicity and cytokine production, which is expressed on all NK cells, a subpopulation of T cells, monocytes and basophils. The 2B4 antigen identified on NK cells and T cells is capable of transmitting stimulatory signals and non-MHC-restricted killing. Reported as an activating receptor, human 2B4 can effectively activate and enhance NK cell–mediated cytotoxicity, induce secretion of IFN-γ and matrix metalloproteinases (MMPs), as well as NK cell invasiveness. As a cell surface glycoprotein of the Ig-superfamily structurally related to CD2-like molecules such as CD2, CD48, CD58, CD84, Ly-9, and SLAM, 2B4 (CD244) is expressed on all human NK cells, a subpopulation of T cells, basophils and monocytes. 2B4 activates NK cell mediated cytotoxicity, induces secretion of IFN-gamma and matrix metalloproteinases, and NK cell invasiveness.
Immune CheckpointImmune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: IP Antibodies Immune Checkpoint Detection: FCM AntibodiesImmune Checkpoint ProteinsImmune Checkpoint Targets Co-inhibitory Immune Checkpoint Targets Immunotherapy Cancer Immunotherapy Targeted Therapy
Full Name
CD244 molecule, natural killer cell receptor 2B4
References
Nakajima H, et al. (2000) 2B4: an NK cell activating receptor with unique specificity and signal transduction mechanism. Hum Immunol. 61(1): 39-43.Chuang SS, et al. (2001) 2B4 (CD244)-mediated activation of cytotoxicity and IFN-gamma release in human NK cells involves distinct pathways. J Immunol. 167(11): 6210-6.McNerney ME, et al. (2005) 2B4 (CD244) is a non-MHC binding receptor with multiple functions on natural killer cells and CD8+ T cells. Mol Immunol. 42(4): 489-94.Mathew SO, et al. (2009) Functional role of human NK cell receptor 2B4 (CD244) isoforms. Eur J Immunol. 39(6): 1632-41.
