Anti-14-3-3 sigma Antibody

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Anti-14-3-3 sigma Antibody (Rabbit Polyclonal 抗体) の製品情報

製品名
Anti-14-3-3 sigma Antibody
検証済のアプリケーション
WB,ELISA,IHC-P,IP
交差反応
Reacts with: Mouse
特異性
Mouse 14-3-3 sigma
免疫原
Recombinant Mouse 14-3-3 sigma / Stratifin / YWHAS / SFN protein (Catalog#50576-MNCE)
調製
Produced in rabbits immunized with purified, recombinant Mouse 14-3-3 sigma / Stratifin / YWHAS / SFN (rM 14-3-3 sigma / Stratifin / YWHAS / SFN; Catalog#50576-MNCE; NP_061224.2; Met 1-Ser 248). 14-3-3 sigma / Stratifin / YWHAS / SFN specific IgG was purified by Mouse 14-3-3 sigma / Stratifin / YWHAS / SFN affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS with 5% trehalose
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-14-3-3 sigma Antibody (Rabbit Polyclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:1000-1:4000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-14-3-3 sigma Antibody (Rabbit Polyclonal 抗体) の画像

Immunochemical staining of mouse SFN in mouse skin with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).

Anti-SFN rabbit polyclonal antibody at 1:500 dilution

Lane A: A431 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:28 kDa

Observed band size:28 kDa

Mouse SFN was immunoprecipitated using:

Lane A:0.5 mg A431 Whole Cell Lysate

Lane B:0.5 mg Hela Whole Cell Lysate

2 µL anti-Mouse SFN rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-Mouse SFN rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 28 kDa

Observed band size: 28 kDa

Anti-14-3-3 sigma Antibody: Alternative Names

Anti-Er Antibody; Anti-Mme1 Antibody; Anti-Ywhas Antibody

14-3-3 sigma Background Information

14-3-3 protein sigma (YWHAS), also known as stratifin (SFN) and epithelial cell marker protein 1, is a member of the14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 1 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2, and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally, YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.
Full Name
stratifin
References
  • Zakut-Houri R., et al.,(1985), Human p53 cellular tumor antigen: cDNA sequence and expression in COS cells. EMBO J. 4:1251-1255.
  • Lamb P., et al., (1986), Characterization of the human p53 gene.Mol. Cell. Biol. 6:1379-1385.
  • Harlow E., et al.,(1985), Molecular cloning and in vitro expression of a cDNA clone for human cellular tumor antigen p53.Mol. Cell. Biol. 5:1601-1610.
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