Anti-14-3-3 eta / YWHAH Antibody

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Anti-14-3-3 eta / YWHAH Antibody (Mouse Monoclonal 抗体) の製品情報

製品名
Anti-14-3-3 eta / YWHAH Antibody
検証済のアプリケーション
WB,ELISA,IP
交差反応
Reacts with: Human
特異性
Human 14-3-3 eta / YWHAH
No cross-reactivity in ELISA with
E.coli cell lysate
免疫原
Recombinant Human 14-3-3 eta / YWHAH protein (Catalog#10847-H09E)
調製
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 14-3-3 eta / YWHAH (rh 14-3-3 eta / YWHAH; Catalog#10847-H09E; Q04917; Gly2-Asn246). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
ソース
Monoclonal Mouse IgG1 Clone #06
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-14-3-3 eta / YWHAH Antibody (Mouse Monoclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:2000
ELISA 1:1000-1:2000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-14-3-3 eta / YWHAH Antibody (Mouse Monoclonal 抗体) の画像

Anti-YWHAH mouse monoclonal antibody at 1:500 dilution

Lane A: U937 Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lane C: Jurkat Whole Cell Lysate

Lane D: K562 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:28 kDa

Observed band size:30 kDa

YWHAH was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

Lane B:0.5 mg k562 Whole Cell Lysate

2 µL anti-YWHAH mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-YWHAH mouse monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 28 kDa

Observed band size: 28 kDa

Anti-14-3-3 eta / YWHAH Antibody: Alternative Names

Anti-YWHA1 Antibody

14-3-3 eta / YWHAH Background Information

YWHAH, the gene encoding the 14-3-3eta isoform, is highly expressed in retinal ganglion cells (RGC). YWHAH is a positional and functional candidate gene for both schizophrenia and bipolar disorder (BP). It is located on chromosome 22q12.3, a region which has been implicated by linkage studies in both BP and schizophrenia.
Full Name
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta
References
  • Wakui H, et al. (1997) Interaction of the ligand-activated glucocorticoid receptor with the 14-3-3 eta protein. J Biol Chem. 272(13): 8153-6.
  • Toyooka K, et al. (2002) Isolation and structure of the mouse 14-3-3 eta chain gene and the distribution of 14-3-3 eta mRNA in the mouse brain. Brain Res Mol Brain Res. 100(1-2): 13-20.
  • Kim YS, et al. (2005) Role of 14-3-3 eta as a positive regulator of the glucocorticoid receptor transcriptional activation. Endocrinology. 146(7): 3133-40.
  • Grover D, et al. (2009) Family-based association of YWHAH in psychotic bipolar disorder. Am J Med Genet B Neuropsychiatr Genet. 150B(7): 977-83.
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