Anti-14-3-3 beta Antibody

Human 14-3-3 beta/YWHAB Immunoprecipitation(IP) 8026
Human 14-3-3 beta/YWHAB Western blot (WB) 13291
Human 14-3-3 beta/YWHAB Immunoprecipitation(IP) 8026
Human 14-3-3 beta/YWHAB Western blot (WB) 13291
Cat: 10843-RP02
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Anti-14-3-3 beta Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-14-3-3 beta Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human 14-3-3 beta
Immunogen
Recombinant Human 14-3-3 beta / YWHAB protein (Catalog#10843-H09E)
Preparation
Produced in rabbits immunized with purified, recombinant Human 14-3-3 beta / YWHAB (rh 14-3-3 beta / YWHAB; Catalog#10843-H09E; NP_003395.1; Met1-Asn246). 14-3-3 beta / YWHAB specific IgG was purified by Human 14-3-3 beta / YWHAB affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-14-3-3 beta Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-14-3-3 beta Antibody (Rabbit Polyclonal antibody) Images
Human 14-3-3 beta/YWHAB Immunoprecipitation(IP) 8026

YWHAB was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-YWHAB rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-YWHAB rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 28 kDa

Observed band size: 28 kDa

Human 14-3-3 beta/YWHAB Western blot (WB) 13291

Anti-YWHAB rabbit polyclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lane B: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:28 kDa

Observed band size:34 kDa

(We are unsure as to the identity of these extra bands.)

Anti-14-3-3 beta Antibody Alternative Names
Anti-GW128 Antibody;Anti-HEL-S-1 Antibody;Anti-HS1 Antibody;Anti-KCIP-1 Antibody;Anti-YWHAA Antibody
14-3-3 beta Background Information

14-3-3 beta / YWHAB is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 beta / YWHAB is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. 14-3-3 beta / YWHAB interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. 14-3-3 beta / YWHAB has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 beta / YWHAB binding negatively regulates RSK1 activity to maintain signal specificity and that association/dissociation of the 14-3-3beta-RSK1 complex is likely to be important for mitogen-mediated RSK1 activation.

Full Name
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta
Research Areas
References
  • Tommerup N, et al. (1996) Assignment of the human genes encoding 14,3-3 Eta (YWHAH) to 22q12, 14-3-3 zeta (YWHAZ) to 2p25.1-p25.2, and 14-3-3 beta (YWHAB) to 20q13.1 by in situ hybridization. Genomics. 33(1): 149-50.
  • Jin YH, et al. (2008) Sirt2 interacts with 14-3-3 beta/gamma and down-regulates the activity of p53. Biochem Biophys Res Commun. 368(3): 690-5.
  • Sekimoto T, et al. (2004) 14-3-3 suppresses the nuclear localization of threonine 157-phosphorylated p27(Kip1). EMBO J. 23(9): 1934-42.
    • Product Description Host Clonality Application Catalog# (PDF)
    Anti-14-3-3 beta Antibody Mouse Monoclonal WB,ELISA,IP 90022-MM05
    Anti-14-3-3 beta Antibody Rabbit Polyclonal WB,IP 90022-T36
    Anti-14-3-3 beta Antibody Mouse Monoclonal ELISA,FCM,ICC/IF,IF 10843-MM03
    Anti-14-3-3 beta Antibody (APC) Mouse Monoclonal FCM 10843-MM03-A
    Anti-14-3-3 beta Antibody Rabbit Polyclonal ELISA 10843-RP01
    Anti-14-3-3 beta Antibody (FITC) Mouse Monoclonal FCM 10843-MM03-F
    Anti-14-3-3 beta Antibody (PE) Mouse Monoclonal FCM 10843-MM03-P
    Anti-14-3-3 beta Antibody (PerCP) Mouse Monoclonal FCM 10843-MM03-C
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