-HB06JU1305-IP.png)
| Application | Dilution | Notes |
|---|---|---|
| WB | 1:500-1:2000 | |
| ELISA | 1:5000-1:10000 | |
| IP | 1-4 μL/mg of lysate |
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
YWHAB was immunoprecipitated using:
Lane A:0.5 mg Hela Whole Cell Lysate
Lane B:0.5 mg HepG2 Whole Cell Lysate
2 µL anti-YWHAB rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:Anti-YWHAB rabbit polyclonal antibody,at 1:200 dilution
Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution
Developed using the odssey technique.Performed under reducing conditions.
Predicted band size: 28 kDaObserved band size: 28 kDa
Anti-YWHAB rabbit polyclonal antibody at 1:500 dilution
Lane A: Hela Whole Cell Lysate
Lane B: HepG2 Whole Cell Lysate
Lysates/proteins at 30 μg per lane.Secondary
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
Developed using the Odyssey technique.Performed under reducing conditions.
Predicted band size:28 kDaObserved band size:34 kDa
(We are unsure as to the identity of these extra bands.)
14-3-3 beta / YWHAB is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 beta / YWHAB is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. 14-3-3 beta / YWHAB interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. 14-3-3 beta / YWHAB has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 beta / YWHAB binding negatively regulates RSK1 activity to maintain signal specificity and that association/dissociation of the 14-3-3beta-RSK1 complex is likely to be important for mitogen-mediated RSK1 activation.
| Product Description | Host | Clonality | Application | Catalog# (PDF) |
|---|---|---|---|---|
| Anti-14-3-3 beta Antibody | Mouse | Monoclonal | WB,ELISA,IP | 90022-MM05 |
| Anti-14-3-3 beta Antibody | Rabbit | Polyclonal | WB,IP | 90022-T36 |
| Anti-14-3-3 beta Antibody | Mouse | Monoclonal | ELISA,FCM,ICC/IF,IF | 10843-MM03 |
| Anti-14-3-3 beta Antibody (APC) | Mouse | Monoclonal | FCM | 10843-MM03-A |
| Anti-14-3-3 beta Antibody | Rabbit | Polyclonal | ELISA | 10843-RP01 |
| Anti-14-3-3 beta Antibody (PerCP) | Mouse | Monoclonal | FCM | 10843-MM03-C |
| Anti-14-3-3 beta Antibody (PE) | Mouse | Monoclonal | FCM | 10843-MM03-P |
| Anti-14-3-3 beta Antibody (FITC) | Mouse | Monoclonal | FCM | 10843-MM03-F |
