Anti-14-3-3 beta Antibody

Cat: 90022-MM05

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Anti-14-3-3 beta Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-14-3-3 beta Antibody
Applications
WB,ELISA,IP
Species reactivity
Reacts with: Cynomolgus
Specificity
Cynomolgus 14-3-3 beta
Has cross-reactivity in ELISA with
Human YWHAB
No cross activity in ELISA with
E.coli cell lysate
Immunogen
Recombinant Cynomolgus 14-3-3 beta / YWHAB protein (Catalog#90022-CNCE)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Cynomolgus 14-3-3 beta / YWHAB (Catalog#90022-CNCE; Q4R572-2; Met 2-Asn 244). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG2b Clone #05
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-14-3-3 beta Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:1000-1:2000  
IP 4-6 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-14-3-3 beta Antibody (Mouse Monoclonal antibody) Images

Cynomolgus 14-3-3 beta/YWHAB Western blot (WB) 13757

Anti-YWHAB mouse monoclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lane B: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:28 kDa

Observed band size:32 kDa

Human CD69 / CLEC2C Immunoprecipitation(IP) 8059

cyno YWHAB was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

Lane B:0.5 mg Hela Whole Cell Lysate

2 µL anti-cyno YWHAB mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-cyno YWHAB mouse monoclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 32 kDa

Observed band size: 32 kDa

14-3-3 beta Background Information

14-3-3 beta / YWHAB is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 beta / YWHAB is found in both plants and mammals, and this protein is 1% identical to the mouse ortholog. 14-3-3 beta / YWHAB interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. 14-3-3 beta / YWHAB has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 beta / YWHAB binding negatively regulates RSK1 activity to maintain signal specificity and that association/dissociation of the 14-3-3beta-RSK1 complex is likely to be important for mitogen-mediated RSK1 activation.
Full Name
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta
References
  • Tommerup N, et al. (1996) Assignment of the human genes encoding 14,3-3 Eta (YWHAH) to 22q12, 14-3-3 zeta (YWHAZ) to 2p25.1-p25.2, and 14-3-3 beta (YWHAB) to 20q13.1 by in situ hybridization. Genomics. 33(1): 149-50.
  • Jin YH, et al. (2008) Sirt2 interacts with 14-3-3 beta/gamma and down-regulates the activity of p53. Biochem Biophys Res Commun. 368(3): 690-5.
  • Sekimoto T, et al. (2004) 14-3-3 suppresses the nuclear localization of threonine 157-phosphorylated p27(Kip1). EMBO J. 23(9): 1934-42.
  • Product Description Host Clonality Application Catalog# (PDF)
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