VSIG4 ELISA Kit, Human

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VSIG4 ELISA Kit, Human: 製品情報

製品名
VSIG4 ELISA Kit, Human
検出の原理
Solid Phase Sandwich ELISA (quantitative)
標識物
HRP
検出する試料
Lysate
感度
12.63 pg/mL
線形範囲
23.44-1500 pg/mL
特異性
Recognizes both recombinant and natural Human VSIG4
干渉
Preparations of APP、FLT4、NEF and GK at 25 ng/mL in a mid-range recombinant human VSIG4 control were assayed for interference. No significant interference was observed.
精度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.22 0.40 0.84
SD 0.02 0.04 0.07
CV(%) 8.2% 10.1% 8.1%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.25 0.46 0.93
SD 0.02 0.05 0.08
CV(%) 7.8% 10.6% 8.1%
回収率
The recovery of Human VSIG4 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=4)
Average % Recovery
85
Range
81-88%
直線性
Supernatant
  Recovery of detected
1:2 83%
1:4 82%
1:8 81%
1:16 88%
ELISAキット(すぐに使用できる)の成分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
製品概要
This VSIG4 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human VSIG4 protein in Lysate . It contains recombinant Human VSIG4, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
配送方法
This ELISA Kit is shipped at ambient temperature.
保存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

VSIG4 ELISA Kit, Human: 画像

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human VSIG4. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

VSIG4 ELISA Kit, Human: 別名

CRIg ELISA Kit, Human; Z39IG ELISA Kit, Human

VSIG4 背景情報

VSIG4 (V-set and immunoglobulin domain containing 4), also known as complement receptor of the immunoglobulin superfamily (CRIg) and Z39Ig, is a type I transmembrane glycoprotein. It is a B7 family-related protein and an Ig superfamily member. In contrast to the B7 family members which contain two IgG domains, VSIG4 contains one complete V-type Ig domain and a truncated C-type Ig domain. VSIG4 is exclusively expressed on tissue resident macrophages and binds to multimers of C3b and iC3b that are covalently attached to particle surfaces. No VSIG4 expression appears to be present in T and B cells. VSIG4 functions as a negative regulator of T cell activation, and may be involved in the maintenance of peripheral T cell tolerance, and is also identified as a potent suppressor of established inflammation. Mouse VSIG4 is synthesized as a 28 amino acid precursor that contains a signal sequence, an V-type Ig domain (aa 36-115), one potential N-linked glycosylation site, and a single transmembrane domain. The V-type Ig domain of mouse VSIG4 shares 86% and 8% aa sequence identity with the V-type Ig domains of rat and human VSIG4, respectively.
完全な名称
V-set and immunoglobulin domain containing 4
参考文献
  • Vogt, L. et al., 2006, J Clin Invest.116: 2817-2826.
  • Helmy, K. et al., 2006, Cell. 124:915-927.
  • Wiesmann, C. et al., 2006, Nature. 444:217-220.
  • Zang,X. et al., 2006, J Clin Invest. 116: 2590-2593.
  • Katschke, KJ. et al., 2007, J. Exp. Med. 204:1319-1325.
  • He,JQ. et al., 2008, Mol Immunol. 45: 4041-4047.
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