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IL-4 Protein, Mouse, Recombinant (Q136D, Y139D, His Tag)

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IL-4 Protein, Mouse, Recombinant (Q136D, Y139D, His Tag): 製品情報

純度
> 95 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
1. Measured by its binding ability in a functional ELISA. Immobilized mouse IL4-His (Cat:51084-M08B) at 10 μg/mL (100 μl/well) can bind rat IL4R-Fc (Cat:80198-R02H), The EC50 of rat IL4R-Fc (Cat:80198-R02H) is 0.12-0.29 μg/mL.
2. Measured by its ability to inhibit mIL4-dependent proliferation of HT-2 cells. The ED50 for this effect is typically 2-10 ng/ml in the presence of 2 ng/ml Recombinant Mouse IL4.
タンパク質の構築
A DNA sequence encoding the mouse IL4 (P07750) (Met 1-Ser140, Q136D, Y139D) was expressed with a polyhistidine tag at the C-terminus.
NCBI Reference No.
発現ホスト
Baculovirus-Insect Cells
Mouse
予測N末端
His 21
分子量
The recombinant mouse IL4 consists of 131 amino acids and predicts a molecular mass of 14.9 KDa. It migrates as an approximately 19 KDa band in SDS-PAGE under reducing conditions due to glycosylation.
バッファー
Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.5.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL-4 Protein, Mouse, Recombinant (Q136D, Y139D, His Tag): 画像

IL-4 Protein, Mouse, Recombinant (Q136D, Y139D, His Tag): 別名

BSF-1 Protein, Mouse; Il-4 Protein, Mouse

IL-4 背景情報

Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and for the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as of other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibites in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and of the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells.
完全な名称
interleukin 4
参考文献
  • Grunewald SM. et al., 1998, J Immunol. 160 (8): 4004-9.
  • Susanne M. et al, 1997, THE JOURNAL OF BIOLOGICAL CHEMISTRY. 272 (3): 1480-3.
  • Nishikubo K. et al., 2003, Gene Ther. 10 (26): 2119-25.
  • Der f 31, a novel allergen from Dermatophagoides farinae, activates epithelial cells and enhances lung-resident group 2 innate lymphoid cells
    Author
    Wang, H;Lin, J;Zeng, L;Ouyang, C;Ran, P;Yang, P;Liu, Z;
    Year
    2017
    Journal
    Sci Rep
  • Pplase of Dermatophagoides farinae promotes ovalbumin-induced airway allergy by modulating the functions of dendritic cells in a mouse model
    Author
    Wang, H;Mo, L;Xiao, X;An, S;Liu, X;Ba, J;Wu, W;Ran, P;Yang, P;Liu, Z;
    Year
    2017
    Journal
    Sci Rep
    Application
    cell-based (culture)
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