RANKL Protein

RANKL Protein Overview

RANKL protein family

Belongs to the tumor necrosis factor family.

RANKL protein name

Recommended name
Tumor necrosis factor ligand superfamily member 11
Aliases
CD254, ODF, OPGL, RANKL, TRANCE
Alternative name
Osteoclast differentiation factor Osteoprotegerin ligand Receptor activator of nuclear factor kappa-B ligand TNF-related activation-induced cytokine CD_antigen: CD254 Tumor necrosis factor ligand superfamily member 11, membrane form Tumor necrosis factor ligand superfamily member 11, soluble form

RANKL Protein Sequence

Species Human RANKL protein
Length 317
Mass (Da) 35478
Sequence Human RANKL protein sequence
Species Mouse RANKL protein
Length 316
Mass (Da) 35003
Sequence Mouse RANKL protein sequence
Species Rat RANKL protein
Length 318
Mass (Da) 35370
Sequence Rat RANKL protein sequence

RANKL Protein Molecular Weight & PI

Tumor necrosis factor ligand superfamily member 11 (Osteoclast differentiation factor) (ODF) (Osteoprotegerin ligand) (OPGL) (Receptor activator of nuclear factor kappa-B ligand) (RANKL) (TNF-related activation-induced cytokine) (TRANCE) (CD254 antigen) [Contains: Tumor necrosis factor ligand superfamily member 11, membrane form; Tumor necrosis factor ligand superfamily member 11, soluble form] Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 1-317 Tumor necrosis factor ligand superfamily

Molecular weight (Da)

35478.11

Theoretical pI

7.24

RANKL Protein Structure

Crystal structure of hRANKL-mRANK complex
Deposited
2015-05-26   Released:  2015-10-14
Deposition Author(s)
Ren, J.
Organism(s)
Homo sapiens, Mus musculus
Expression System
Escherichia coli BL21(DE3)
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.8000 Å
R-Value Free
0.203
R-Value Work
0.171
5BNQ From PDB

Human RANKL protein Secondary structure

RANKL Protein Interaction

Recombinant RANKL Protein Feature

RANKL Protein, Human, Recombinant (Fc Tag)

High Purity
> 90 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
1. Immobilized mouse mFc-TNFSF11 at 10 μg/ml (100 μl/well) can bind biotinylated human TNFRSF11B-His (Cat:10271-H08H), The EC50 of biotinylated human TNFRSF11B-His (Cat:10271-H08H) is 6.0-14.1 ng/ml. 2. The bioactivity of hRANKL was determined by measuring the ability of hRANKL to induce TRAP activity in Raw 264.7 cells. The ED50 for this effect is typically 15-45 ng/mL.

Recombinant RANKL protein citations

Title
Factors that Affect the Osteoclastogenesis of RAW264. 7 Cells
Year
2017
Author
Nguyen, J;Nohe, A;
Journal
J Biochem Analyt Stud
Title
Factors that Affect the Osteoclastogenesis of RAW264.7 Cells
Year
2017
Author
Nguyen, J;Nohe, A;
Journal
J Biochem Anal Stud

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