クイック注文

qEASY qPCR Primers are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human, mouse, rat genomes. It can be widely applied in the quantitative analysis of gene expression.

Unique primer design   Strict validation process   High specificity & sensitivity   Provide customized service
qPCR primer pairs:multiple species, validated in positive organizations
 
human qPCR primer pairs
6000+ human qPCR primer pairs, lyophilized qPCR  primer mix, 2nmol, containing positive organization validation report
  mouse qPCR primer pairs
4000+ mouse qPCR primer pairs, lyophilized qPCR  primer mix, 2nmol, containing positive organization validation report
 
rat qPCR primer pairs
2000+ rat qPCR primer pairs, lyophilized qPCR  primer mix, 2nmol, containing positive organization validation report
 
qPCR primers of 96-well plates:multiple signaling pathways, metabolic pathways, cancer detections, etc., provide customized service

Features & Advantages

  • Unique primer design
    — To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
  • Strict validation process
    — Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
  • Uniform PCR conditions, saving time and cost.
  • ~100% amplification efficiency, ensuring the accuracy of the RNA quantitative.
human qPCR primer pairs mouse qPCR primer pairs rat qPCR primer pairs

qPCR primer pairs Introduction

qEASY qPCR Primer Pairs are designed using SBI's proprietary primer design algorithm. They are used for SYBR Green dye-based real-time PCR and designed according to the conserved region of all variants of a specific gene. At least one primer crosses the junction of adjacent exons to avoid amplification of genomic DNA directly and effectively. Our primer pairs cover all genes from human, mouse, rat and can be widely applied to the quantitative analysis of gene expression. cDNA used as templates, a single, correct-size band is produced in SYBR Green dye-based PCR with each pair of primers. Therefore, our primers have the characteristics with high specificity, high amplification efficiency, wide linear range and uniform reaction conditions. Each package for a specific gene is supplied with a lyophilized mixture of forward and reverse primers that can be used directly in SYBR Green dye-based real-time PCR after they are dissolved into ultrapure water.

qPCR primer pairs Validation Data

45 pairs of gene-specific qPCR primers as examples,positive tissue cDNA and gene standard validation

  • Amplification curves: amplification tendency enhanced
  • Melting curves: high specificity
  • Electrophoresis detection: high specificity
  • Standard curve: high sensitivity, detectable limit is less than 100 copies, high amplification efficiency, wide linear range
   
a. Amplification curves of 45 primer
pairs in cDNA
  b. Melting curves of 45 primer pairs in
cDNA
  e. Amplification curves of SDHA
standard curve
 
   
c. Agarose gel electrophoresis
validation result
  d. Amplification efficiency of 45 primer
pairs in plasmid
  f. Standard curve of SDHA

45 pairs of gene-specific qPCR primers were experimentally validated by SYBR Green method using first-strand cDNA and ddH2O as template. There were no non-specific amplification and primer-dimers produced by melting curve analysis and agarose gel electrophoresis detection. We used a dilution series (10-fold dilutions) of a purified plasmid as templates to construct standard curves for primer by SYBR Green-based real-time RT-PCR. We drew the standard curve by SYBR Green method in the qPCR experiment. The result showed that the qPCR Primers detectable limit is less than 100 copies and the amplification efficiency is between 90% ~ 105%. a. Amplification curves of 45 primer pairs in cDNA. (cDNA and ddH2O as templates);b. Melting curves of 45 primer pairs corresponding with a; c. Agarose gel electrophoresis validation result corresponding with a, the odd number lane is cDNA template detection, the even number lane is the corresponding NTC (No Template Control) results; d. Statistical graph of amplification efficiency about 45 primer pairs with a dilution series plasmid as templates; e. Amplification curves of SDHA standard curve; f. Standard curve of SDHA, the amplification efficiency is 99.0%.

Hot qPCR Primers

Gene Catalog# (PDF) Product Description Availability
GAPDH HP100003 human GAPDH qPCR primer pairs In stock
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IL1B HP100210 human IL1B qPCR primer pairs In stock
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IL6 HP100427 human IL6 qPCR primer pairs In stock
CD274 MP200010 mouse CD274 qPCR primer pairs In stock
TNF HP100592 human TNF qPCR primer pairs In stock
PFKFB3 HP102593 Human PFKFB3 qPCR primer pairs In stock
CD274 HP100170 human CD274 qPCR primer pairs In stock
FAP HP101274 human FAP qPCR primer pairs In stock

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Product description

  • • Price: $79
  • • Lyophilized qPCR  primer mix, 1 nmol each primer, for ~200 PCR reactions
  • • Availability: Delivery of In-stock on the next day, others for 5-8 business days
  • • 12,000+ Primers based on our cDNAs
  • • The quantity RT-PCR protocol and verification report are listed in the COA

Hot qPCR primer pairs

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