PD-L1 Protein, Human, Recombinant (His Tag)

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PD-L1 Protein, Human, Recombinant (His Tag): 製品情報

純度
> 98 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
Measured by its binding ability in a functional ELISA . Immobilized human B7-H1 at 20 μg/ml (100 μl/well) can bind human PD1 with a linear ranger of 0.032-0.8 μg/ml .
タンパク質の構築
A DNA sequence encoding the N-terminal segment (Met 1-Thr 239) of the extracellular domain of human B7-H1 (NP_054862.1) was expressed with a C-terminal polyhistidine tag.
NP No.
発現ホスト
HEK293 Cells
Human
予測N末端
Phe 19
分子量
The recombinant mature human B7-H1 comprises 232 amino acids and predicts a molecular mass of 26.8 kDa. As a result of glycosylation, the rh B7-H1 migrates as an approximately 35-38 kDa protein in SDS-PAGE under reducing conditions.
バッファー
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PD-L1 Protein, Human, Recombinant (His Tag): 画像

Measured by its binding ability in a functional ELISA . Immobilized human B7-H1 at 20 μg/ml (100 μl/well) can bind human PD1 with a linear ranger of 0.032-0.8 μg/ml

PD-L1 Protein, Human, Recombinant (His Tag): 別名

B7-H Protein, Human; B7-H1 Protein, Human; B7H1 Protein, Human; PD-L1 Protein, Human; PDCD1L1 Protein, Human; PDCD1LG1 Protein, Human; PDL1 Protein, Human

PD-L1 背景情報

Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.
完全な名称
CD274 molecule
参考文献
  • Iwai Y, et al. (2002) Involvement of PD-L1 on tumor cells in the escape from host immune system and tumor immunotherapy by PD-L1 blockade. Proc Natl Acad Sci U S A. 99(19): 12293-7.
  • Ghebeh H, et al. (2006) The B7-H1 (PD-L1) T lymphocyte-inhibitory molecule is expressed in breast cancer patients with infiltrating ductal carcinoma: correlation with important high-risk prognostic factors. Neoplasia. 8(3): 190-8.
  • Salih HR, et al. (2006) The role of leukemia-derived B7-H1 (PD-L1) in tumor-T-cell interactions in humans. Exp Hematol. 34(7): 888-94.
  • Wilcox RA, et al. (2009) B7-H1 (PD-L1, CD274) suppresses host immunity in T-cell lymphoproliferative disorders. Blood. 114(10): 2149-58.
  • Ruggiero A, et al. (2009) Crystal structure of PD-L1, a ribosome inactivating protein from Phytolacca dioica L. leaves with the property to induce DNA cleavage. Biopolymers. 91(12): 1135-42.
  • Design, isolation and evaluation of the binding efficiency of a DNA aptamer against interleukin 2 receptor alpha, in vitro
    Author
    Shahdordizadeh, M;Taghdisi, SM;Sankian, M;Ramezani, M;Abnous, K;
    Year
    2017
    Journal
    Int. Immunopharmacol.
  • Non-invasive assessment of murine PD-L1 levels in syngeneic tumor models by nuclear imaging with nanobody tracers
    Author
    Broos, K;Keyaerts, M;Lecocq, Q;Renmans, D;Nguyen, T;Escors, D;Liston, A;Raes, G;Breckpot, K;Devoogdt, N;
    Year
    2017
    Journal
    Oncotarget
  • Novel Humanized Monoclonal Antibodies to PD-L1
    Author
    Simonetti, M;Alton, K;Shimkets, R;
    Year
    2017
    Journal
    Abeome Intelligent Antibody Discovery
    Application
    animal-based
  • Selection of DNA aptamers against Mycobacterium tuberculosis Ag85A, and its application in a graphene oxide-based fluorometric assay
    Author
    Ansari, N;Ghazvini, K;Ramezani, M;
    Year
    2018
    Journal
    Microchimica Acta
  • A secreted PD-L1 splice variant that covalently dimerizes and mediates immunosuppression
    Author
    Mahoney, KM;Shukla, SA;Patsoukis, N;Chaudhri, A;Browne, EP;Arazi, A;Eisenhaure, TM;Pendergraft, WF;Hua, P;Pham, HC;Bu, X;Zhu, B;Hacohen, N;Fritsch, EF;Boussiotis, VA;Wu, CJ;Freeman, GJ;
    Year
    2018
    Journal
    Cancer Immunol. Immunother.
    Application
    SDS-PAGE and WB (control)
  • PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 Pathways
    Author
    Zhao, Y;Lee, CK;Lin, CH;Gassen, RB;Xu, X;Huang, Z;Xiao, C;Bonorino, C;Lu, LF;Bui, JD;Hui, E;
    Year
    2019
    Journal
    Immunity
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