慢病毒载体 cDNA ORF 克隆

Lentiviral ORF Clone / Plasmid

Lentivirus, a type of retrovirus, has become the most popular gene transduction tool. Lentiviral vectors can effectively infect neuronal cells, hepatocytes, cardiomyocytes, tumor cells, endothelial cells, stem cells, and other types of cells. For some difficult-to-transfect cells, such as primary cells and non-differentiated cells, the use of lentiviral vectors can greatly increase the transduction efficiency of the target gene. Lentivirus also has obvious advantages in transient transfection and stable strain screening.
In order to meet different experimental needs, Sinobiological provides various forms of lentiviral ORF clone / plasmid with high-efficiency transfection and better biosafety for customers.

Free trial activity of high quality lentiviral ORF clone / plasmid is in progress!
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You can browse molecules, species, etc. in the attachment. After receiving your trial application, we will contact with you in time.
Attachment:Trial list of lenti-ORF clones
◇ Activity time?
From now until 30 June 2018
 
 
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As of June 15, 2018:
127 people have applied for trial!!
 
Data is updated weekly
 

Features of lentiviral ORF clone / plasmid

◇ High-efficiency transfection 慢病毒载体示意图 ◇ Better biosafety
Transfected dividing or non-dividing cells, transient or stable expression The third generation lentivirus vector, which contains specific elements to improve transgene expression, virus titer, biosafety
◇ Full length sequence confirmed ◇ Multiple species
Full length sequence confirmed, quality assurance Nearly 40,000 molecules covering human, mice, rats and other species to meet specific needs
◇ Wide Compatibility ◇ Multiple tags
Transfer plasmid can be freely combined with other companies' package kit, all can obtain high titer lentivirus particles • Untagged
• C terminal:Myc, HA, FLAG, His, GFPSpark® and OFPSpark®
• N terminal:Myc, HA, FLAG, His, GFPSpark® and OFPSpark®

Advantages of lentiviral ORF clone / plasmid

◇ Lentiviral Titer Test

慢病毒滴度测定图 慢病毒滴度测定图 慢病毒滴度测定图 慢病毒滴度测定图 慢病毒滴度测定图
pLV-GFPSpark® plasmid (Cat. No.: LVCV-01) was transfected into 293LT cells and viral fluid was collected 48 hours later. The lentivirus titer was up to 1.27x10^8 TU/ml measured by flow cytometry.

◇ Lentiviral Particles Infect Different Cells

293FT(MOI=1) MCF7(MOI=5) HepG2(MOI=20) HT1080 (MOI=6) MC38 (MOI=150)
慢病毒颗粒按照不同MOI 侵染不同细胞 慢病毒颗粒按照不同MOI 侵染不同细胞 慢病毒颗粒按照不同MOI 侵染不同细胞 慢病毒颗粒按照不同MOI 侵染不同细胞 慢病毒颗粒按照不同MOI 侵染不同细胞
GFPSpark® lentiviral particles infected different cells at different multiplicity of infection (MOI), 72 hours later, observed under fluorescent microscope.

◇ Wide Compatibility

pLV-GFPSpark® (SB) +
Package Kit (A company)
pLV-GFPSpark® (SB)+
Package Kit (B company)
义翘转移质粒具有广泛兼容性 义翘转移质粒具有广泛兼容性
The transfer plasmid can be freely combined with other companies' package kit, all can obtain high titer lentivirus particles. A:pLV-GFPSpark® (SB) + Package Kit (A company);B:pLV-GFPSpark® (SB)+ Package Kit (B company)

Types of lentiviral vectors

• pLV-C-Flag • pLV-N-Flag • pLV-SP-N-Flag
• pLV-C-His • pLV-N-His • pLV-SP-N-His
• pLV-C-HA • pLV-N-HA • pLV-SP-N-HA
• pLV-C-Myc • pLV-N-Myc • pLV-SP-N-Myc
• pLV-C-OFPSpark® • pLV-N-OFPSpark® • pLV-Untagged
• pLV-C-GFPSpark® • pLV-N-GFPSpark®  

Partial list of lentiviral ORF clone / plasmid

More Lentiviral ORF clone / plasmid reagentsand you can search for Lentiviral ORF clone / plasmid with multiple tags directly

Molecule Catalog Tag Species
PD-L1/B7-H1/CD274 HG10084-ACGLN C-GFPSpark® Human
PD-L2/B7-DC/CD273 HG10292-ACGLN C-GFPSpark® Human
CD80/B7-1 HG10698-ACGLN C-GFPSpark® Human
CD86/B7-2 HG10699-ACGLN C-GFPSpark® Human
CD28/TP44 HG11524-ACGLN C-GFPSpark® Human
CTLA-4/CD152 HG11159-ACGLN C-GFPSpark® Human
VEGF/VEGFA HG11066-ACGLN C-GFPSpark® Human
HER2/ERBB2 HG10004-ACGLN C-GFPSpark® Human
ERBB3/HER3 HG10201-ACGLN C-GFPSpark® Human
VEGFR2/KDR/Flk-1/CD309 HG10012-ACGLN C-GFPSpark® Human
TNF-alpha/TNFA/TNFSF2 HG10602-ACGLN C-GFPSpark® Human
TP53/p53 HG10182-ACGLN C-GFPSpark® Human
ANG1/ANGPT1 HG13667-ACGLN C-GFPSpark® Human

More information about lentiviral vector