IL-1 beta Protein, Human, Recombinant (pro form, His Tag)

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IL-1 beta Protein, Human, Recombinant (pro form, His Tag): 製品情報

純度
> 95 % as determined by SDS-PAGE
内毒素
Please contact us for more information.
活性
1. Measured by its binding ability in a functional ELISA.
2. Immobilized human IL1B at 10 μg/mL (100 μl/well) can bind human IL1R1. The EC50 of human IL1R1 is 0.198 μg/mL.
タンパク質の構築
A DNA sequence encoding the pro form of human IL1β (NP_000567.1) (Met 1-Ser 269) was fused with a polyhistidine tag at the N-terminus.
NP No.
発現ホスト
E. coli
Human
予測N末端
Met
分子量
The recombinant pro form of human IL1β consisting of 280 amino acids and has a calculated molecular mass of 32.3 kDa. It migrates as an approximately 34 kDa band in SDS-PAGE under reducing conditions.
バッファー
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL-1 beta Protein, Human, Recombinant (pro form, His Tag): 画像

1. Measured by its binding ability in a functional ELISA. 2. Immobilized human IL1B at 10 μg/mL (100 μl/well) can bind human IL1R1. The EC50 of human IL1R1 is 0.198 μg/mL.

IL-1 beta Protein, Human, Recombinant (pro form, His Tag): 別名

IL-1 Protein, Human; IL-1 beta Protein, Human; IL-1B Protein, Human; IL1-BETA Protein, Human; IL1F2 Protein, Human

IL-1 beta 背景情報

Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL1 superfamily consists of three members, IL1A (IL1 alpha), IL1B (IL1 beta), and IL1 receptor antagonist (IL1Ra). In clinical, it has been reported that Interleukin (IL)-1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of successful pregnancy. Proinflammatory interleukin (IL)-1 gene polymorphisms associated with high levels of IL-1beta activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc).
完全な名称
interleukin 1, beta
参考文献
  • Kim SH, et al. (2008) Association of -31TC and -511 CT polymorphisms in the interleukin 1 beta (IL1B) promoter in Korean keratoconus patients. Mol Vis. 14:2109-16.
  • Wang ZC, et al. (2002) T helper 1-type immunity to trophoblast antigens in women with a history of recurrent pregnancy loss is associated with polymorphism of the IL1B promoter region. Genes Immun. 3(1): 38-42.
  • Mattuzzi S, et al. (2007) Association of polymorphisms in the IL1B and IL2 genes with susceptibility and severity of systemic sclerosis. J Rheumatol. 34(5): 997-1004.
  • Intracellular and extracellular regulation of the inflammatory protease caspase-1
    Author
    OR Shamaa
    Year
    2014
    Journal
    Thesis
    Application
    substrate
  • Acid-dependent Interleukin-1 (IL-1) Cleavage Limits Available Pro-IL-1β for Caspase-1 Cleavage
    Author
    Edye, ME;Brough, D;Allan, SM;
    Year
    2015
    Journal
    J. Biol. Chem.
    Application
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    Ainscough, JS;Gerberick, GF;Kimber, I;Dearman, RJ;
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    2015
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    J. Biol. Chem.
    Application
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    Author
    Shamaa, OR;Mitra, S;Gavrilin, MA;Wewers, MD;
    Year
    2015
    Journal
    PLoS ONE
    Application
    substrate
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    Author
    Ainscough, JS;Macleod, T;McGonagle, D;Brakefield, R;Baron, JM;Alase, A;Wittmann, M;Stacey, M;
    Year
    2017
    Journal
    Proc. Natl. Acad. Sci. U.S.A
    Application
    substrate
  • Matrix Metalloproteinase-9-Dependent Release of IL-1β by Human Eosinophils
    Author
    Esnault, S;Kelly, E;Johnson, S;DeLain, L;Haedt, M;Noll, A;Sandbo, N;Jarjour, N;
    Year
    2019
    Journal
    Mediators of Inflammation
    Application
    substrate
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