VEGFR2 / KDR Matched ELISA Antibody Pair Set,Human

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VEGFR2 / KDR Matched ELISA Antibody Pair Set,Human 製品情報

製品名
VEGFR2 / KDR Matched ELISA Antibody Pair Set,Human
検出原理
Solid Phase Sandwich ELISA
特異性
Quantitative determination of Human VEGFR2 / KDR
線形範囲
25-1600 pg/mL
ELISA 抗体ペアセット(すぐに使用できません)の成分
1. Capture Antibody 1 mg/mL of rabbit anti-Human VEGFR2/KDR/Flk-1/CD309 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10012-R001)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human VEGFR2/KDR/Flk-1/CD309 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 55 ng of recombinant Human VEGFR2/KDR/Flk-1/CD309. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1600 pg/mL is recommended.
製品概要
This VEGFR2 / KDR Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human VEGFR2 / KDR . It contains Human VEGFR2 / KDR capture antibody, Human VEGFR2 / KDR detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human VEGFR2 / KDR protein. This Pair Set is at affordable price for researchers.
配送方法
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
保存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

VEGFR2 / KDR Matched ELISA Antibody Pair Set,Human の画像

VEGFR2 / KDR Matched ELISA Antibody Pair Set,Human: 別名

CD309 Matched ELISA Antibody Pair Set, Human; Flk-1 Matched ELISA Antibody Pair Set, Human; FLK1 Matched ELISA Antibody Pair Set, Human; VEGFR Matched ELISA Antibody Pair Set, Human; VEGFR2 Matched ELISA Antibody Pair Set, Human

VEGFR2 / KDR 背景情報

VEGFR2, also called as KDR or Flk-1, is identified as the receptor for VEGF and VEGFC and an early marker for endothelial cell progenitors, whose expression is restricted to endothelial cells in vivo. VEGFR2 was shown to be the primary signal transducer for angiogenesis and the development of pathological conditions such as cancer and diabetic retinopathy. It has been shown that VEGFR2 is expressed mainly in the endothelial cells, and the expression is upregulated in the tumor vasculature. Thus the inhibition of VEGFR2 activity and its downstream signaling are important targets for the treatment of diseases involving angiogenesis. VEGFR2 transduces the major signals for angiogenesis via its strong tyrosine kinase activity. However, unlike other representative tyrosine kinase receptors, VEGFR2 does not use the Ras pathway as a major downstream signaling but rather uses the phospholipase C-protein kinase C pathway to signal mitogen-activated protein (MAP)-kinase activation and DNA synthesis. VEGFR2 is a direct and major signal transducer for pathological angiogenesis, including cancer and diabetic retinopathy, in cooperation with many other signaling partners; thus, VEGFR2 and its downstream signaling appear to be critical targets for the suppression of these diseases. VEGF and VEGFR2-mediated survival signaling is critical to endothelial cell survival, maintenance of the vasculature and alveolar structure and regeneration of lung tissue. Reduced VEGF and VEGFR2 expression in emphysematous lungs has been linked to increased endothelial cell death and vascular regression.
完全な名称
kinase insert domain receptor
研究分野
参考文献
  • Shibuya M. (2006) Vascular endothelial growth factor (VEGF)-Receptor2: its biological functions, major signaling pathway, and specific ligand VEGF-E. Endothelium. 13(2): 63-9.
  • Marwick JA, et al. (2010) Cigarette smoke regulates VEGFR2-mediated survival signaling in rat lungs. J Inflamm (Lond). 7(1): 11.
  • Bruns AF, et al. (2010) Ligand-stimulated VEGFR2 signaling is regulated by co-ordinated trafficking and proteolysis. Traffic. 11(1): 161-74.
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