RANKL Matched ELISA Antibody Pair Set,Human

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RANKL Matched ELISA Antibody Pair Set,Human 製品情報

製品名
RANKL Matched ELISA Antibody Pair Set,Human
検出原理
Solid Phase Sandwich ELISA
特異性
Quantitative determination of Human RANKL
線形範囲
234.38-15000 pg/mL
ELISA 抗体ペアセット(すぐに使用できません)の成分
1. Capture Antibody 0.5 mg/mL of rabbit anti-Human RANKL / OPGL / TNFSF11 / CD254 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 11682-R128)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human RANKL / OPGL / TNFSF11 / CD254 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.1 μg/mL in detection antibody dilution buffer before use. (Catalog: # 11682-R101)
3. Standard Each vial contains 570 ng of recombinant Human RANKL / OPGL / TNFSF11 / CD254. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 15000 pg/mL is recommended.
製品概要
This RANKL Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human RANKL . It contains Human RANKL capture antibody, Human RANKL detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human RANKL protein. This Pair Set is at affordable price for researchers.
配送方法
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
保存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

RANKL Matched ELISA Antibody Pair Set,Human の画像

RANKL Matched ELISA Antibody Pair Set,Human: 別名

CD254 Matched ELISA Antibody Pair Set, Human; hRANKL2 Matched ELISA Antibody Pair Set, Human; ODF Matched ELISA Antibody Pair Set, Human; OPGL Matched ELISA Antibody Pair Set, Human; OPTB2 Matched ELISA Antibody Pair Set, Human; RANKL Matched ELISA Antibody Pair Set, Human; sOdf Matched ELISA Antibody Pair Set, Human; TRANCE Matched ELISA Antibody Pair Set, Human

RANKL 背景情報

Tumor necrosis factor ligand superfamily member 11, also known as Receptor activator of nuclear factor kappa-B ligand, Osteoprotegerin ligand, TNFSF11, RANKL, TRANCE, OPGL and CD254, is a single-pass type II membrane protein which belongs to the tumor necrosis factor family. The receptor activator of nuclear factor-kappaB ligand (RANKL), its cognate receptor RANK, and its natural decoy receptor osteoprotegerin have been identified as the final effector molecules of osteoclastic bone resorption. RANK and RANKL are key regulators of bone remodeling and regulate T cell/dendritic cell communications, and lymph node formation. Moreover, RANKL and RANK are expressed in mammary gland epithelial cells and control the development of a lactating mammary gland during pregnancy. Genetically, RANKL and RANK are essential for the development and activation of osteoclasts and bone loss in response to virtually all triggers tested. Inhibition of RANKL function via the natural decoy receptor osteoprotegerin (OPG, TNFRSF11B) prevents bone loss in postmenopausal osteoporosis and cancer metastases. Importantly, RANKL appears to be the pathogenetic principle that causes bone and cartilage destruction in arthritis. RANK-RANKL signaling not only activates a variety of downstream signaling pathways required for osteoclast development, but crosstalk with other signaling pathways also fine-tunes bone homeostasis both in normal physiology and disease. In addition, RANKL and RANK have essential roles in lymph node formation, establishment of the thymic microenvironment, and development of a lactating mammary gland during pregnancy.
完全な名称
tumor necrosis factor (ligand) superfamily, member 11
参考文献
  • Takayanagi H, et al. (2002) Signaling crosstalk between RANKL and interferons in osteoclast differentiation. Arthritis Res. 4 Suppl 3: S227-32.
  • Nakashima T, et al. (2003) RANKL and RANK as novel therapeutic targets for arthritis. Curr Opin Rheumatol. 15(3): 280-7.
  • Schwarz EM, et al. (2007) Clinical development of anti-RANKL therapy. Arthritis Res Ther. 9 Suppl 1: S7.
  • Leibbrandt A, et al. (2008) RANK/RANKL: regulators of immune responses and bone physiology. Ann N Y Acad Sci. 1143: 123-50.
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