15-PGDH ELISA Kit, Human

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15-PGDH ELISA Kit, Human: 製品情報

製品名
15-PGDH ELISA Kit, Human
検出の原理
Solid Phase Sandwich ELISA (quantitative)
標識物
HRP
検出する試料
Lysate
感度
19.2 pg/mL
線形範囲
54.69-3500 pg/mL
特異性
Recognizes both recombinant and natural Human 15-PGDH
干渉
Preparations of huaman APP at 20 ng/mL in a mid-range recombinant human HPGD control were assayed for interference. No significant interference was observed.
精度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 453.80 829.92 1,712.26
SD 14.95 24.66 54.59
CV(%) 3.3% 3.0% 3.2%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 486.65 954.63 1,965.70
SD 28.54 109.99 219.87
CV(%) 5.9% 11.5% 11.2%
ELISAキット(すぐに使用できる)の成分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
製品概要
This 15-PGDH ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human 15-PGDH protein in Lysate . It contains recombinant Human 15-PGDH, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
配送方法
This ELISA Kit is shipped at ambient temperature.
保存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

15-PGDH ELISA Kit, Human: 画像

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

15-PGDH ELISA Kit, Human: 別名

15-PGDH ELISA Kit, Human; PGDH ELISA Kit, Human; PGDH1 ELISA Kit, Human; PHOAR1 ELISA Kit, Human; SDR36C1 ELISA Kit, Human

15-PGDH 背景情報

15-hydroxyprostaglandin dehydrogenase [NAD+], also known as Prostaglandin dehydrogenase 1, HPGD, and PGDH1, is a member of the short-chain dehydrogenases/reductases (SDR) family. Prostaglandins (PGs) play a key role in the onset of labor inmany species and regulate uterine contractility and cervicaldilatation. Therefore, the regulation of prostaglandin outputby PG synthesizing and metabolizing enzymes in the human myometrium may determine uterine activitypatterns in human labor both at preterm and at term. Prostaglandin dehydrogenase (PGDH) metabolizes prostaglandins (PGs) to render them inactive. HPGD is down-regulated by cortisol, dexamethasone and betamethasone and down-regulated in colon cancer. It is up-regulated by TGFB1. HPGD contributes to the regulation of events that are under the control of prostaglandin levels. HPGD catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. and inhibits in vivo proliferation of colon cancer cells. Defects in HPGD are the cause of primary hypertrophic osteoathropathy autosomal recessive (PHOAR), cranioosteoarthropathy (COA),and isolated congenital nail clubbing.
完全な名称
hydroxyprostaglandin dehydrogenase 15-(NAD)
参考文献
  • Patel, FA. et al., 2003, J. Clin. Endocrinol. Metab. 88: 2922-33.
  • McKeown KJ, et al.,2003, J. Clin. Endocrinol. Metab. 88 (4): 1737-41.
  • Yan, M. et al., 2004, Proc. Natl. Acad. Sci. USA. 101: 17468-73.
  • Tariq, M. et al., 2009, J Med Genet. 46 (1): 14-20.
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