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人々 Coagulation Factor X/F10 Gene ORF cDNA clone expression plasmid

データシートレビュー関連製品プロトコル
Human F10 cDNA クローン製品情報
Gene_bank_ref_id:NM_000504.3
cDNA サイズ:1461bp
cDNA の説明:Full length Clone DNA of Homo sapiens coagulation factor X.
遺伝子の別名:F10, FX, FXA
:Human
ベクター:pCMV/hygro
Plasmid:pCMV-F10
制限サイト:HindIII + XbaI (5.5kb + 1.46kb)
タグ シーケンス:
シーケンスの説明:Identical with the Gene Bank Ref. ID sequence except for the point mutation: 792 C/T not causing the amino acid variation.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
Human F10 Gene Plasmid Map
Human F10 / FX Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
pCMV/hygro Vector Information
 
Vector Name pCMV/hygro
Vector Size 5657bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive ,Stable / Transient
Promoter CMV
Antibiotic Resistance Ampicillin
Selection In Mammalian Cells Hygromycin
Protein Tag None
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)


Schematic of pCMV/hygro Multiple Cloning Sites
人々 Coagulation Factor X/F10 Gene ORF cDNA clone expression plasmid on other vectors
人々 Coagulation Factor X/F10 Gene ORF cDNA clone expression plasmid, C-GFPSpark タグHG11076-ACGJPY54410
人々 Coagulation Factor X/F10 Gene ORF cDNA clone expression plasmid, C-OFPSpark タグHG11076-ACRJPY54410
人々 Coagulation Factor X/F10 Gene ORF cDNA clone expression plasmid, N-GFPSpark タグHG11076-ANGJPY54410
人々 Coagulation Factor X/F10 Gene ORF cDNA clone expression plasmid, N-OFPSpark タグHG11076-ANRJPY54410
人々 Coagulation Factor X/F10 Gene ORF cDNA clone expression plasmid, C-Flag タグHG11076-CFJPY47150
人々 Coagulation Factor X/F10 Gene ORF cDNA clone expression plasmid, C-His タグHG11076-CHJPY47150
人々 Coagulation Factor X/F10 Gene ORF cDNA clone expression plasmid, C-Myc タグHG11076-CMJPY47150
人々 Coagulation Factor X/F10 Gene ORF cDNA clone expression plasmid, C-HA タグHG11076-CYJPY47150
人々 Coagulation Factor X/F10 Gene ORF cDNA clone in cloning vectorHG11076-GJPY18140
人々 Coagulation Factor X/F10 Gene ORF cDNA clone expression plasmidHG11076-G-NJPY47150
人々 Coagulation Factor X/F10 Gene ORF cDNA clone expression plasmid, N-Flag タグHG11076-NFJPY47150
人々 Coagulation Factor X/F10 Gene ORF cDNA clone expression plasmid, N-His タグHG11076-NHJPY47150
人々 Coagulation Factor X/F10 Gene ORF cDNA clone expression plasmid, N-Myc タグHG11076-NMJPY47150
人々 Coagulation Factor X/F10 Gene ORF cDNA clone expression plasmid, N-HA タグHG11076-NYJPY47150
人々 Coagulation Factor X/F10 Gene ORF cDNA clone expression plasmidHG11076-UTJPY47150
 発現ベクターの詳細情報
Product nameProduct name
背景

Coagulation factor X, also known as FX, F10, Eponym Stuart-Prower factor, and thrombokinase, is an enzyme of the coagulation cascade. It is one of the vitamin K-dependent serine proteases, and plays a crucial role in the coagulation cascade and blood clotting, as the first enzyme in the common pathway of thrombus formation. Factor X deficiency is one of the rarest of the inherited coagulation disorders. FX deficiency among the most severe of the rare coagulation defects, typically including hemarthroses, hematomas, and umbilical cord, gastrointestinal, and central nervous system bleeding. Factor X is synthesized in the liver as a mature heterodimer formed from a single-chain precursor, and vitamin K is essential for its synthesis. Factor X is activated into factor Xa (FXa) by both factor IX (with its cofactor, factor VIII in a complex known as intrinsic Xase) and factor VII (with its cofactor, tissue factor in a complex known as extrinsic Xase) through cleaving the activation propeptide. As the first member of the final common pathway or thrombin pathway, FXa converts prothrombin to thrombin in the presence of factor Va, Ca2+, and phospholipid during blood clotting and cleaves prothrombin in two places (an arg-thr and then an arg-ile bond). This process is optimized when factor Xa is complexed with activated cofactor V in the prothrombinase complex. Inborn deficiency of factor X is very uncommon, and may present with epistaxis (nose bleeds), hemarthrosis (bleeding into joints) and gastrointestinal blood loss. Apart from congenital deficiency, low factor X levels may occur occasionally in a number of disease states. Furhermore, factor X deficiency may be seen in amyloidosis, where factor X is adsorbed to the amyloid fibrils in the vasculature.

参考文献
  • Rosen ED. (2002) Gene targeting in hemostasis. Factor X. Front Biosci. 7: d1915-25.
  • Uprichard J, et al. (2002) Factor X deficiency. Blood Rev. 16(2): 97-110.
  • Borensztajn K, et al. (2008) Factor Xa: at the crossroads between coagulation and signaling in physiology and disease. Trends Mol Med. 14(10): 429-40.
  • Menegatti M, et al. (2009) Factor X deficiency. Semin Thromb Hemost. 35(4): 407-15.
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    カタログ: HG11076-G-N
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    • Human F10 / FX Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
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