CD55 / DAF ELISA Kit, Human

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CD55 / DAF ELISA Kit, Human: 製品情報

製品名
CD55 / DAF ELISA Kit, Human
検出の原理
Solid Phase Sandwich ELISA (quantitative)
標識物
HRP
検出する試料
Serum
感度
91.65 pg/mL
線形範囲
125-8000 pg/mL
特異性
Recognizes both recombinant and natural Human CD55 / DAF
精度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 1,915.32 3,714.11 6,592.55
SD 42.23 98.04 284.36
CV(%) 2.2% 2.6% 4.3%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 1,483.40 3,332.00 6,295.20
SD 37.20 77.29 371.11
CV(%) 2.5% 2.3% 5.9%
回収率
The recovery of Human CD55 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
84
Range
82 -86%
直線性
Serum
  Recovery of detected
1:2 92%
1:4 92%
1:8 89%
1:16 91%
ELISAキット(すぐに使用できる)の成分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
製品概要
This CD55 / DAF ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CD55 / DAF protein in Serum . It contains recombinant Human CD55 / DAF, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
配送方法
This ELISA Kit is shipped at ambient temperature.
保存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

CD55 / DAF ELISA Kit, Human: 画像

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human CD55. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

CD55 / DAF ELISA Kit, Human: 別名

CR ELISA Kit, Human; CROM ELISA Kit, Human; DAF ELISA Kit, Human; TC ELISA Kit, Human

CD55 / DAF 背景情報

CD55, also well known as decay-accelerating factor (DAF), is a member of the RCA (regulators of complement activation) family characterized by four to 3 SCRs (short consensus repeats) in their plasma-exposed regions. It is a major regulator of the alternative and classical pathways of complement activation and is expressed on all serum-exposed cells. CD55 is physiologically acting as an inhibitor of the complement system, but is also broadly expressed in malignant tumours. DAF seems to exert different functions beyond its immunological role such as promotion of tumorigenesis, decrease of complement mediated tumor cell lysis, autocrine loops for cell rescue and evasion of apoptosis, neoangiogenesis, invasiveness, cell motility. It is commonly hijacked by invading pathogens, including many enteroviruses and uropathogenic Escherichia coli, to promote cellular attachment prior to infection. This 7-75 kDa glycoprotein CD55 containing four SCR modules is involved in the regulation of the complement cascade. It inhibits complement activation by suppressing the function of C3/C5 convertases, thereby limiting local generation or deposition of C3a/C5a and membrane attack complex (MAC or C5b-9) production. DAF has been identified as a ligand for an activation-associated, seven-transmembrane lymphocyte receptor, CD97, which is a receptor mediating attachment and infection of several viruses and bacteria. In addition, it has been shown that DAF regulates the interplay between complement and T cell immunity in vivo, and thus may be implicated in immune and tumor biology.
完全な名称
CD55 molecule, decay accelerating factor for complement (Cromer blood group)
参考文献
  • Lea S. (2002) Interactions of CD55 with non-complement ligands. Biochem Soc Trans. 30(Pt 6): 1014-9.
  • Mikesch JH, et al. (2006) The expression and action of decay-accelerating factor (CD55) in human malignancies and cancer therapy. Cell Oncol. 28(5-6): 223-32.
  • Wang Y, et al. (2010) Decay accelerating factor (CD55) protects neuronal cells from chemical hypoxia-induced injury. J Neuroinflammation. 7:24.
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