Recombinant human CD33 (AAH28152.1, Met1-His259) are conjugated with PE under optimum conditions, the unreacted PE was removed.
The PE to protein molar ratio is 0.1-1
Aqueous solution containing 0.5% BSA and 0.03%Proclin 300
10 μl/Test, 0.1 mg/ml
Form & Shipping:
Liquid. Shipping at ambient temperature.
This reagent is stable for 6 months from date of receipt when stored at 2℃ - 8℃. Protected from prolonged exposure to light. Do not freeze !
The 5×10E5 cells are suspended in 50 μL washing buffer (1 X PBS + 1% BSA), add 10 μL Protein L-PE, incubate for 45 min at 4℃ from light, then wash 2 times with wash buffer. Analyze by flow cytometry immediately.
Flow cytometric analysis of anti-CD33 CAR expression. 293 cells were lentivirally transduced with anti-CD33 CAR. Flow cytometric analysis was performed with PE-conjugated recombinant human CD33 (Cat. No. 12238-HCCH-P) . Non-transduced 293 cells were used as a control (left).
Human CD33 / Siglec-3 Protein (PE conjugated): 別名
p67; Siglec-3; SIGLEC3
Myeloid cell surface antigen CD33 also known as Sialic acid binding Ig-like lectin 3, CD33 antigen or Siglec-3, is a member of the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. This Single-pass type I membrane protein contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain. CD33 /Siglec-3 is a putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. CD33 /Siglec-3 preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33/Siglec-3 induces apoptosis in acute myeloid leukemia (in vitro). CD33/Siglec-3 can function as a sialic acid-dependent cell adhesion molecule and that binding can be modulated by endogenous sialoglycoconjugates when CD33 is expressed in a plasma membrane.