Human CD33 / Siglec-3 Protein (PE conjugated)

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General Information
Expression System:

HEK293 Cells

Preparation:

Recombinant human CD33 (AAH28152.1, Met1-His259) are conjugated with PE under optimum conditions, the unreacted PE was removed.

Labeling Rate:

The PE to protein molar ratio is 0.1-1

Formulation:

Aqueous solution containing 0.5% BSA and 0.03%Proclin 300

Concentration:

10 μl/Test, 0.1 mg/ml

Form & Shipping:

Liquid. Shipping at ambient temperature.

Storage:

This reagent is stable for 6 months from date of receipt when stored at 2℃ - 8℃. Protected from prolonged exposure to light. Do not freeze !

Usage:

The 5×10E5 cells are suspended in 50 μL washing buffer (1 X PBS + 1% BSA), add 10 μL Protein L-PE, incubate for 45 min at 4℃ from light, then wash 2 times with wash buffer. Analyze by flow cytometry immediately.

Test results

Flow cytometric analysis of anti-CD33 CAR expression. 293 cells were lentivirally transduced with anti-CD33 CAR. Flow cytometric analysis was performed with PE-conjugated recombinant human CD33 (Cat. No. 12238-HCCH-P) . Non-transduced 293 cells were used as a control (left).

Human CD33 / Siglec-3 Protein (PE conjugated): 別名

p67; Siglec-3; SIGLEC3

CD33 背景情報

Myeloid cell surface antigen CD33 also known as Sialic acid binding Ig-like lectin 3, CD33 antigen or Siglec-3, is a member of the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. This Single-pass type I membrane protein contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain. CD33 /Siglec-3 is a putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. CD33 /Siglec-3 preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33/Siglec-3 induces apoptosis in acute myeloid leukemia (in vitro). CD33/Siglec-3 can function as a sialic acid-dependent cell adhesion molecule and that binding can be modulated by endogenous sialoglycoconjugates when CD33 is expressed in a plasma membrane.
完全な名称
CD33 molecule
参考文献
  • Simmons D, et al. (1988) Isolation of a cDNA encoding CD33, a differentiation antigen of myeloid progenitor cells. J Immunol. 141(8): 2797-800.
  • Ulyanova T, et al. (1999) The sialoadhesin CD33 is a myeloid-specific inhibitory receptor. Eur J Immunol. 29(11): 3440-9.
  • Freeman SD, et al. (1995) Characterization of CD33 as a new member of the sialoadhesin family of cellular interaction molecules. Blood. 85(8): 2005-12.
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