Dengue virus 4 (DENV-4) (strain Philippines/H241/1956) DENV-NS1 Gene ORF cDNA clone expression plasmid, C-Flag tag

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Dengue virus 4 (DENV-4) (strain Philippines/H241/1956) DENV-NS1 Gene ORF cDNA clone expression plasmid, C-Flag tag: 製品情報

遺伝子
DENV
NCBI 参考シーケンス番号
遺伝子の長さ
1101 bp
製品の特徴
Full length Clone DNA of Dengue virus 4 (DENV4) (strain Philippines/H241/1956) DENV-NS1 with C terminal Flag tag.
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
制限部位
KpnI + XbaI(6kb+1.1kb)
タグシーケンス
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Kanamycin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.
Note: Flag® is a registered trademark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

DENV-NS1 cDNA ORF ヌクレオチド配列およびアミノ酸配列に関する情報

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Dengue virus 4 (DENV-4) (strain Philippines/H241/1956) DENV-NS1 Gene ORF cDNA clone expression plasmid, C-Flag tag: 検証済の画像

The plasmid was transfected into 293E adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.

Dengue virus 4 (DENV-4) (strain Philippines/H241/1956) DENV-NS1 Gene ORF cDNA clone expression plasmid, C-Flag tag: 別名

DENV-NS1 cDNA ORF Clone, DENV

DENV-NS1 cDNA ORFクローン, DENV, 関連製品

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