CD16a Protein, Human, Recombinant (176 Phe, His Tag)

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CD16a Protein, Human, Recombinant (176 Phe, His Tag): 製品情報

純度
> 97 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
Measured by its binding ability in a functional ELISA. Immobilized human CD16a-His (Cat:10389-H08H) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.90-2.30 μg/ml.
タンパク質の構築
A DNA sequence encoding the extracellular domain (Met 1-Gln 208) of human CD16a (P08637-1) precursor was expressed with a C-terminal polyhistidine tag. It is identical to FCGR3A158F/V in the reference.
NCBI Reference No.
発現ホスト
HEK293 Cells
Human
予測N末端
Gly 17
分子量
The secreted recombinant human CD16a consists of 203 amino acids and has a predicted molecular mass of 23.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh CD16a is approximately 45-50 kDa due to glycosylation.
バッファー
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD16a Protein, Human, Recombinant (176 Phe, His Tag): 画像

CD16a Protein, Human, Recombinant (176 Phe, His Tag): 別名

CD16 Protein, Human; CD16A Protein, Human; Fc gamma RIIIa Protein, Human; FCG3 Protein, Human; FCGR3 Protein, Human; FCGRIII Protein, Human; FCR-10 Protein, Human; FCRIII Protein, Human; FCRIIIA Protein, Human; IGFR3 Protein, Human; IMD20 Protein, Human

CD16a 背景情報

The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells. CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.
完全な名称
Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
参考文献
  • David Dornan, et al. Effect of FCGR2A and FCGR3A variants on CLL outcome. Blood. 2010 116: 4212-4222
  • Fc Gamma Receptor Glycosylation Modulates the Binding of IgG Glycoforms: A Requirement for Stable Antibody Interactions
    Author
    Hayes, JM;Frostell, A;Cosgrave, EF;Struwe, WB;Potter, O;Davey, GP;Karlsson, R;Anneren, C;Rudd, PM;
    Year
    2014
    Journal
    J. Proteome Res.
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