Anti-Carboxypeptidase A Antibody

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Anti-Carboxypeptidase A Antibody (Rabbit Polyclonal 抗体) の製品情報

製品名
Anti-Carboxypeptidase A Antibody
検証済のアプリケーション
WB,ELISA,IHC-P,IP
交差反応
Reacts with: Human
特異性
Human Carboxypeptidase A
免疫原
Recombinant Human Carboxypeptidase A1 / CPA1 protein (Catalog#10504-H08H)
調製
Produced in rabbits immunized with purified, recombinant Human Carboxypeptidase A1 / CPA1 (rh Carboxypeptidase A1 / CPA1; Catalog#10504-H08H; NP_001859.1; Met 1-Tyr 419). Carboxypeptidase A1 / CPA1 specific IgG was purified by Human Carboxypeptidase A1 / CPA1 affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Carboxypeptidase A Antibody (Rabbit Polyclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:2500-1:10000
IP 4-6 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Carboxypeptidase A Antibody (Rabbit Polyclonal 抗体) の画像

Immunochemical staining of human CPA1 in human pancreas with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).

CPA1 was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

2 µL anti-CPA1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CPA1 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 50 kDa

Observed band size: 50 kDa

Anti-CPA1 rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 60 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:55 kDa

Observed band size:47 kDa

(We are unsure as to the identity of these extra bands.)

Anti-Carboxypeptidase A Antibody: 別名

Anti-CPA Antibody

Carboxypeptidase A 背景情報

Carboxypeptidase A1 (CPA1)is secreted as a pancreatic procarboxypeptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group, with the preference of residues with aromatic or branched aliphatic side chains. CPA1 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E, and is involved in zymogen inhibition. Three different forms of human pancreatic procarboxypeptidase A have been isolated.
完全な名称
carboxypeptidase A1 (pancreatic)
参考文献
  • Catasus, L. et al., 1992, Biochem. J. 287: 299-303.
  • Moulard, M. et al., 1990, FEBS. Lett. 261: 179-183.
  • Aloy, P. et al., 1998, Biol. Chem. 379: 149-155.

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