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Biotinylated protein

what are biotinylated protein ?

In biochemistry, biotinylation is the process of covalently attaching biotin to a protein. Biotinylation is rapid, specific and is unlikely to perturb the natural function of the molecule due to the small size of biotin (MW = 244.31 g/mol). Biotin binds to streptavidin and avidin with an extremely high affinity, fast on-rate, and high specificity, and these interactions are exploited in many areas of biotechnology to isolate biotinylated molecules of interest.

Application

Purification:The biotin tag can be used in affinity chromatography together with a column that has avidin (or streptavidin or Neutravidin) bound to it, which is the natural ligand for biotin.

Detection:This tag can also be used in detection of the protein via anti-biotin antibodies or avidin/streptavidin-tagged detection strategies such as enzyme reporters or fluorescent probes. This can be useful in localization by fluorescent or electron microscopy,ELISA assays, ELISPOT assays, western blots and other immunoanalytical methods. Detection with monovalent streptavidin can avoid clustering or aggregation of the biotinylated target.

Biotinylated protein Superiority

• Quality control : Activity assay

Activity assay has been done on every recombinant biotinylated proteins. The assay ensure that the activity of biotinylated protein is not influenced by biotinylation.

• Cover most drug targets

Sino Biological Inc. has developed almost all the hot drug target proteins. A large range of biotinylated proteins can be produced as you demand.
For customization, contact cro-service@sinobiological.com.

• Most favorable price

Nearly 6000+ recombinant proteins are in stock. With excellent production technology, prime cost is much lower. All the biotinylated protein are at the most favorable price.

Part of biotinylated protein list

Human   Human   Rhesus   Mouse   Human    176Val   Human    176Phe   Human   Human
B7-DC
(10292-H08H-B)


tag: C-His
Biotinylated
  CD140b
(10514-H08H-B)


tag: His & AVI
Biotinylated
  CD16
(50036-M27H-B)


tag: His & AVI
Biotinylated
  CD16-2
(50036-M27H-B)


tag: C-His
Biotinylated
  CD16a
(10389-H27H1-B)


tag: His & AVI
Biotinylated
  CD16a
(10389-H27H-B)


tag: His & AVI
Biotinylated
  CD16b
(11046-HNAE-B)


tag: Native
Biotinylated
  CD27
(10292-H08H-B)


tag: C-His
Biotinylated
 
Human   Mouse   Mouse   Human    167 His   Human    167 Arg   Cynomolgus   Human   Cynomolgus
CD276
(11188-H08H-B)


tag: C-His
Biotinylated
  CD276
(50973-M08H-B)


tag: C-His
Biotinylated
  CD32
(50030-M27H-B)


tag: His & AVI
Biotinylated
  CD32a
(10374-H27H1-B)


tag: His & AVI
Biotinylated
  CD32a
(10374-H27H-B)


tag: His & AVI
Biotinylated
  CD32a
(90015-C27H-B)


tag: His & AVI
Biotinylated
  CD32b
(10259-H27H-B)


tag: His & AVI
Biotinylated
  CD32b
(90014-C27H-B)


tag: His & AVI
Biotinylated
 
Human   Human   Human   Human   Mouse   Human   Human   Mouse
CD33
(12238-H08H-B)


tag: C-His
Biotinylated
  CD40L
(10239-H08E-B)


tag: C-His
Biotinylated
  CD64
(10256-H08S-B)


tag: C-His
Biotinylated
  CD64
(10256-H27H-B)


tag: His & AVI
Biotinylated
  CD64
(50086-M27H-B)


tag: His & AVI
Biotinylated
  CD8A
(10980-H08H-B)


tag: C-His
Biotinylated
  CD8B
(11031-HCCH-B)


tag: C-cleavage
Biotinylated
  CSF1R
(50059-M08H-B)


tag: His & AVI
Biotinylated
 
Human   Human   Human   Human   Influenza   Human   Human   Human
CTLA4
(11159-H08H-B)


tag: C-His
Biotinylated
  EGFR
(10001-H08H-B)


tag: C-His
Biotinylated
  EpCAM
(10694-H08H-B)


tag: C-His
Biotinylated
  FAP
(10464-H07H-B)


tag: C-His
Biotinylated
  H5N1
(40064-V07H-B)


tag: C-His
Biotinylated
  HER2
(10004-H08H-B)


tag: C-His
Biotinylated
  HER3
(10201-H08H-B)


tag: C-His
Biotinylated
  IFNAR2
(10359-H08H-B)


tag: C-His
Biotinylated
 
Human   Human   Human   Human   Human   Human   Mouse   *****
MSLN
(13128-H01H-B)


tag: Fc
Biotinylated
  PD-L1
(10084-H08H-B)


tag: C-His
Biotinylated
  S100P
(12635-HNAE-B)


tag: Native
Biotinylated
  TNFRSF4
(10481-H08H-B)


tag: C-His
Biotinylated
  VEGF
(10012-H08H-B)


tag: C-His
Biotinylated
  VEGFR2
(10012-H08H-B)


tag: C-His
Biotinylated
  VEGFR2
(50998-M08H-B)


tag: C-His
Biotinylated
  More...
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tag: ***
Biotinylated

References

1. Viens, A.; Harper, F.; Pichard, E.; Comisso, M.; Pierron, G.; Ogryzko, V. (2008). "Use of Protein Biotinylation in Vivo for Immunoelectron Microscopic Localization of a Specific Protein Isoform". Journal of Histochemistry and Cytochemistry. 56 (10): 911–9.
2. Howarth, Mark; Chinnapen, Daniel J-F; Gerrow, Kimberly; Dorrestein, Pieter C; Grandy, Melanie R; Kelleher, Neil L; El-Husseini, Alaa; Ting, Alice Y (2006). "A monovalent streptavidin with a single femtomolar biotin binding site". Nature Methods. 3 (4): 267–73.

注意:すべての製品は、"研究目的のみに使用するものであり、診断または治療目的に使用するものではありません"