Best Seller ! Anti-His Tag Monoclonal Antibody

Anti-His Tag Monoclonal Antibody (105327-MM02) is a reliable tool for the detection of His-tagged target proteins. Sino Biological His Antibody is validated by Western Blot, and can recognize 6× His proteins or 10× His proteins.

HIGHLIGHT

Broad spectrum   High sensitivity   Different positons   Multi-expression systems
Identifies more his-tag proteins   Detects lower expression levels   Recognizes His tags at N-terminal, C-terminal and internal   Ideal for mammalian cells, insect cells and bacteria

 Broad spectrum: Identifies more his-tag proteins

Sino Biological   Competitor A   Competitor B
   
A: insect cell lysate containing His-LYPLA2 protein (0.5μg)      C: HEK293 cell lysate containing His-AKR1C2 protein (20ng)
B: insect cell lysate containing His-AKR1C2 protein (0.5μg)      D: HEK293 cell lysate containing His-LILRA3 protein (200ng)

 High sensitivity: Detects lower expression levels

IL2Ra-His   AZGP1-His
Sino Biological   Sino Biological
 
Competitor A   Competitor A
 
A-E: Different loading quantitiy of IL2Ra-His protein sample   A-E: Different loading quantitiy of AZGP1-His protein sample

 Different positons: Recognizes His tags at N-terminal, C-terminal and internal

N-terminal His Tag   C-terminal His Tag   internal His Tag
   
A: His-Mouse CLEC7A
B: His-Human LYPLA2
C: His-Human COCH
  A: Human ALPL-His
B: Human IL2Ra-His
C: Human IL6R-His
  A: TrxA-His-EK-Human APOE
B: TrXA-His-EK-Human BCL6
C: TrxA-His-EK-Human SOCS3

 Multi-expression systems: Ideal for mammalian cells, insect cells and bacteria

mammalian cells   bacteria   insect cells
   
A: Negative control
B: His-Human LYPLA2 Lysate
C: His-Human AKR1C2 Lysate
D: Human LILRA3-His Lysate
  A: Negative control
B: Human M2E1-His Lysate
C: His-sSPA Lysate
D: His-Human GH1 Lysate
  A: Negative control
B: His-Human LYPLA2 Lysate
C: Human AZGP1-His Lysate
D: Human IL2Ra-His Lysate