TNF-alpha ELISA Kit, Human

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TNF-alpha ELISA Kit, Human: 製品情報

製品名
TNF-alpha ELISA Kit, Human
検出の原理
Solid Phase Sandwich ELISA (quantitative)
標識物
HRP
検出する試料
Cell supernatant
感度
11.63 pg/mL
線形範囲
23.44-1500 pg/mL
特異性
Recognizes both recombinant and natural Human TNF-alpha
干渉
Preparations of the following factors at 50 ng/mL in a mid-range recombinant Human TNFa control were assayed for interference. No significant interference was observed.
精度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 277.00 595.00 1,115.00
SD 10.66 14.44 30.07
CV(%) 3.9% 2.4% 2.7%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 269.00 587.00 1,091.00
SD 20.77 43.24 66.66
CV(%) 7.7% 7.4% 6.1%
回収率
The recovery of Human TNF spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=3)
Average % Recovery
86
Range
73 -95%
直線性
Supernatant
  Recovery of detected
1:2 84%
1:4 109%
1:8 91%
1:16 84%
ELISAキット(すぐに使用できる)の成分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
製品概要
This TNF-alpha ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human TNF-alpha protein in Cell supernatant . It contains recombinant Human TNF-alpha, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
配送方法
This ELISA Kit is shipped at ambient temperature.
保存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

TNF-alpha ELISA Kit, Human: 画像

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human TNFa. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

TNF-alpha ELISA Kit, Human: 別名

DIF ELISA Kit, Human; TNF-alpha ELISA Kit, Human; TNFA ELISA Kit, Human; TNFSF2 ELISA Kit, Human

TNF-alpha 背景情報

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD12a; p55/6) and TNF-R2 (TNF receptor type 2; CD12b; p75/8), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.
氏名
tumor necrosis factor
研究分野
参考文献
  • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
  • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
  • Roles of extrahepatic lipolysis and the disturbance of hepatic fatty acid metabolism in TNF-α -induced hepatic steatosis
    Author
    Yang, R;Guan, MJ;Zhao, N;Li, MJ;Zeng, T;
    Year
    2018
    Journal
    Toxicology
    Application
    cell-based; ELISA
  • LncRNA-MEG3 alleviates high glucose induced inflammation and apoptosis of retina epithelial cells via regulating miR-34a/SIRT1 axis
    Author
    Tong, P;Peng, QH;Gu, LM;Xie, WW;Li, WJ;
    Year
    2018
    Journal
    Exp. Mol. Pathol.
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