Anti-Smad5 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Smad5 Antibody
Validated applications
ELISA,IHC-P
Species reactivity
Reacts with: Mouse
Immunogen
Recombinant Mouse SMAD5 protein (Catalog#50728-M20B)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse SMAD5 (rM SMAD5; Catalog#50728-M20B; P97454; Thr2-Ser465). SMAD5 specific IgG was purified by Mouse SMAD5 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Smad5 Antibody (Rabbit Polyclonal antibody) Validated Applications
| Application |
Dilution |
Notes |
| ELISA |
1:5000-1:10000 |
|
| IHC-P |
1:500-1:2000 |
|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-Smad5 Antibody (Rabbit Polyclonal antibody) Images
Immunochemical staining of mouse SMAD5 in mouse intestine with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Anti-Smad5 Antibody Alternative Names
Anti-1110051M15Rik Antibody;Anti-AI451355 Antibody;Anti-Dwf-C Antibody;Anti-Madh5 Antibody;Anti-MusMLP Antibody
Smad5 Background Information
SMAD5 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD5 is involved in the TGF-beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. It is also involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's). The binding of ligands causes the oligomerization and phosphorylation of the SMAD5 protein. SMAD5 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.
Full Name
SMAD family member 5
References
Vinayagam A. et al., 2011, Sci Signal. 4 (189): rs8. Sangadala S. et al., 2007, J Biomol Struct Dyn. 25 (1): 11-23. Riggins GJ. et al., 1996, Nat Genet. 13 (3): 347-9.
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