Anti-S100A5 Antibody

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Anti-S100A5 Antibody (Mouse Monoclonal 抗体) の製品情報

製品名
Anti-S100A5 Antibody
検証済のアプリケーション
ELISA,ICC/IF
交差反応
Reacts with: Human
特異性
Human S100A5
No cross-reactivity in ELISA with
E.coli cell lysate
免疫原
Recombinant Human S100A5 protein (Catalog#11142-H07E)
調製
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human S100A5 (rh S100A5; Catalog#11142-H07E; B48219; Met 1-Lys 110). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
ソース
Monoclonal Mouse IgG1 Clone #04
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-S100A5 Antibody (Mouse Monoclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
ELISA 1:1000-1:2000
ICC-IF 1:20-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-S100A5 Antibody (Mouse Monoclonal 抗体) の画像

Immunofluorescence staining of Human S100A5 in A431 or Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human S100A5 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Anti-S100A5 Antibody: 別名

Anti-S100D Antibody

S100A5 背景情報

S1 protein?is a family of low molecular weight protein found in vertebrates characterized by two?EF-hand calcium-binding motifs. There are at least 21 different S1 proteins, and the name is derived from the fact that the protein is?1%?soluble in ammonium sulfate?at neutral?pH. Most S1 proteins are disulfide-linked homodimer, and is normally present in cells derived from the?neural crest, chondrocytes, macrophages, dendritic cells, etc. S1 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response.?? Protein S1-A5, also known as Protein S-1D, S1 calcium-binding protein A5, S1A5 and S1D, is a member of the S1 family which contains two?EF-hand domains. S1A5 is also a novel member of the EF-hand superfamily of calcium-binding proteins that is poorly characterized at the protein level. It is expressed in very restricted regions of the adult brain. From birth onwards, S1A5 remained a neuronal-specific protein, only located in a subpopulation of neurons in the spiral ganglion.
完全な名称
S100 calcium binding protein A5
参考文献
  • Sch?fer,B.W. et al., 2000, J Biol Chem. 275 (39):30623-30.
  • Gebhardt, C. et al., 2006, Biochem Pharmacol. 72 (11):1622-31.
  • Nonaka, D. et al., 2008, J. Cutan. Pathol. 35 (11): 1014-9.
  • Lim, SY. et al., 2008, J Immunol. 181 (8): 5627-36.
  • Heibeck TH. et al., 2009, J. Proteome Res. 8:3852-61.

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