|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rat THY1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80337-ACG|
|Rat THY1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80337-ACR|
|Rat THY1 ORF mammalian expression plasmid, C-Flag tag||RG80337-CF|
|Rat THY1 ORF mammalian expression plasmid, C-His tag||RG80337-CH|
|Rat THY1 ORF mammalian expression plasmid, C-Myc tag||RG80337-CM|
|Rat THY1 ORF mammalian expression plasmid, C-HA tag||RG80337-CY|
|Rat THY1 Gene cDNA clone plasmid||RG80337-G|
|Rat THY1 ORF mammalian expression plasmid, N-Flag tag||RG80337-NF|
|Rat THY1 ORF mammalian expression plasmid, N-His tag||RG80337-NH|
|Rat THY1 ORF mammalian expression plasmid, N-Myc tag||RG80337-NM|
|Rat THY1 ORF mammalian expression plasmid, N-HA tag||RG80337-NY|
|Rat THY1 natural ORF mammalian expression plasmid||RG80337-UT|
Mouse Thy-1 membrane glycoprotein, also known as Thy-1 antigen, CD90 and THY1, is a cell membrane protein which contains 1 Ig-like V-type (immunoglobulin-like) domain. It is a glycophosphatidylinositol-linked glycoprotein expressed on the surface of neurons, thymocytes, subsets of fibroblasts, endothelial cells, mesangial cells and some hematopoietic cells. It has been identified on a variety of stem cells and at varying levels in non-lymphoid tissues such as on fibroblasts, brain cells, and activated endothelial cells. Thy-1 is evolutionarily conserved, developmentally regulated, and often has dramatic effects on cell phenotype. Thy-1 is a 25-37 kDa glycosylphosphatidylinositol (GPI)-anchored protein involved in T cell activation, neurite outgrowth, apoptosis, tumor suppression, wound healing, and fibrosis. To mediate these diverse effects, Thy-1 participates in multiple signaling cascades. Thy-1 is an important regulator of cell-cell and cell-matrix interactions, with important roles in nerve regeneration, metastasis, inflammation, and fibrosis.