|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rat SERPINA1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80476-ACG|
|Rat SERPINA1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80476-ACR|
|Rat SERPINA1 ORF mammalian expression plasmid, C-Flag tag||RG80476-CF|
|Rat SERPINA1 ORF mammalian expression plasmid, C-His tag||RG80476-CH|
|Rat SERPINA1 ORF mammalian expression plasmid, C-Myc tag||RG80476-CM|
|Rat SERPINA1 ORF mammalian expression plasmid, C-HA tag||RG80476-CY|
|Rat SERPINA1 ORF mammalian expression plasmid, N-Flag tag||RG80476-NF|
|Rat SERPINA1 ORF mammalian expression plasmid, N-His tag||RG80476-NH|
|Rat SERPINA1 ORF mammalian expression plasmid, N-Myc tag||RG80476-NM|
|Rat SERPINA1 ORF mammalian expression plasmid, N-HA tag||RG80476-NY|
|Rat SERPINA1 Gene cDNA clone plasmid||RG80476-U|
|Rat SERPINA1 natural ORF mammalian expression plasmid||RG80476-UT|
SerpinA1, also known as Alpha-1 antitrypsin (AAT), is a prototype member of the Serpin superfamily of the serine protease inhibitors. This serine protease inhibitor blocks the protease, neutrophil elastase. Alpha-1 antitrypsin is mainly produced in the liver and acts as an antiprotease. Its principal function is to inactivate neutrophil elastase, preventing tissue damage. SerpinA1 (alpha1-antitrypsin), an acute phase protein and the classical neutrophil elastase inhibitor, is localized within lipid rafts in primary human monocytes in vitro. It association with monocytes is inhibited by cholesterol depleting/efflux-stimulating agents (nystatin, filipin, MbetaCD (methyl-beta-cyclodextrin) and oxidized low-density lipoprotein (oxLDL) and conversely, enhanced by free cholesterol. Furthermore, SerpinA1/monocyte association per se depletes lipid raft cholesterol as characterized by the activation of extracellular signal-regulated kinase 2, formation of cytosolic lipid droplets, and a complete inhibition of oxLDL uptake by monocytes. Previous population studies have suggested that heterozygote status for the AAT gene (SerpinA1) is a risk factor for chronic rhinosinusitis with nasal polyposis (CRSwNP). Alpha-1 antitrypsin deficiency is a recently identified genetic disease that occurs almost as frequently as cystic fibrosis. It is caused by various mutations in the SerpinA1 gene, and has numerous clinical implications. Alpha-1 antitrypsin deficiency is an inherited disease affecting the lung and liver. In the liver, alpha-1 antitrypsin deficiency may manifest as benign neonatal hepatitis syndrome; a small percentage of adults develop liver fibrosis, with progression to cirrhosis and hepatocellular carcinoma. Its most important physiologic functions are the protection of pulmonary tissue from aggressive proteolytic enzymes and regulation of pulmonary immune processes.