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ラット CD45/PTPRC transcript variant 1 Gene ORF cDNA clone expression plasmid, N-His タグ

データシートレビュー関連製品プロトコル
Rat PTPRC cDNA クローン製品情報
Gene_bank_ref_id:NM_001109890.1
cDNA サイズ:3426bp
cDNA の説明:Full length Clone DNA of Rattus norvegicus protein tyrosine phosphatase, receptor type, C, transcript variant 1 with N terminal His tag.
遺伝子の別名:Lca, RT7, CD45, L-CA, T200, Ptprc
:Rat
ベクター:pCMV3-N-His
Plasmid:
制限サイト:
タグ シーケンス:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

ラット CD45/PTPRC transcript variant 1 Gene ORF cDNA clone expression plasmid, N-His タグ on other vectors
ラット CD45/PTPRC transcript variant 1 Gene ORF cDNA clone expression plasmid, C-GFPSpark タグRG80296-ACGJPY78585
ラット CD45/PTPRC transcript variant 1 Gene ORF cDNA clone expression plasmid, C-OFPSpark タグRG80296-ACRJPY78585
ラット CD45/PTPRC transcript variant 1 Gene ORF cDNA clone expression plasmid, N-GFPSpark タグRG80296-ANGJPY78585
ラット CD45/PTPRC transcript variant 1 Gene ORF cDNA clone expression plasmid, N-OFPSpark タグRG80296-ANRJPY78585
ラット CD45/PTPRC transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag タグRG80296-CFJPY71331
ラット CD45/PTPRC transcript variant 1 Gene ORF cDNA clone expression plasmid, C-His タグRG80296-CHJPY71331
ラット CD45/PTPRC transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Myc タグRG80296-CMJPY71331
ラット CD45/PTPRC transcript variant 1 Gene ORF cDNA clone expression plasmid, C-HA タグRG80296-CYJPY71331
ラット CD45/PTPRC transcript variant 1 Gene ORF cDNA clone in cloning vectorRG80296-GJPY18135
ラット CD45/PTPRC transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Flag タグRG80296-NFJPY71331
ラット CD45/PTPRC transcript variant 1 Gene ORF cDNA clone expression plasmid, N-His タグRG80296-NHJPY71331
ラット CD45/PTPRC transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Myc タグRG80296-NMJPY71331
ラット CD45/PTPRC transcript variant 1 Gene ORF cDNA clone expression plasmid, N-HA タグRG80296-NYJPY71331
ラット CD45/PTPRC transcript variant 1 Gene ORF cDNA clone expression plasmidRG80296-UTJPY71331
 発現ベクターの詳細情報
Product nameProduct name
背景

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Protein tyrosine phosphatase, receptor type C (CD45), also known as PTPRC is a member of the protein tyrosine phosphatase (PTP) family which is known for its function to serve as signaling molecules and to regulate a variety of cellular processes such as cell proliferation, differentiation, mitotic cycle and oncogenic transformation. CD45 is found expression specifically in hemotopietic cells. CD45 consists of an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It serves as an essential regulator of T-cell and B-cell antigen receptor signaling through either direct interaction with components of the antigen receptor complexs or by activating various Src family kinases required for the antigen receptor signaling and it also can suppress JAK kinases.

参考文献
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Irie-Sasaki J, et al. (2001) CD45 is a JAK phosphatase and negatively regulates cytokine receptor signaling. Nature. 409: 349-54.
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    カタログ: RG80296-NH
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