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ラット OSTC/oligosaccharyltransferase complex subunit Gene ORF cDNA clone expression plasmid, N-Myc タグ

データシートレビュー関連製品プロトコル
Rat OSTC cDNA クローン製品情報
Gene_bank_ref_id:NM_001108566.1
cDNA サイズ:450bp
cDNA の説明:Full length Clone DNA of Rattus norvegicus oligosaccharyltransferase complex subunit (non-catalytic) with N terminal Myc tag.
遺伝子の別名:Dc2, RGD1560708
:Rat
ベクター:pCMV3-SP-N-Myc
Plasmid:
制限サイト:
タグ シーケンス:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

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ラット OSTC/oligosaccharyltransferase complex subunit Gene ORF cDNA clone expression plasmid, N-Flag タグRG80717-NFJPY47150
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ラット OSTC/oligosaccharyltransferase complex subunit Gene ORF cDNA clone expression plasmid, N-Myc タグRG80717-NMJPY47150
ラット OSTC/oligosaccharyltransferase complex subunit Gene ORF cDNA clone expression plasmid, N-HA タグRG80717-NYJPY47150
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ラット OSTC/oligosaccharyltransferase complex subunit Gene ORF cDNA clone expression plasmidRG80717-UTJPY47150
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カタログ: RG80717-NM
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