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ラット ADP-sugar Pyrophosphatase/NUDT5 Gene ORF cDNA clone expression plasmid, C-Flag タグ

データシートレビュー関連製品プロトコル
Rat NUDT5 cDNA クローン製品情報
Gene_bank_ref_id:NM_001007733.1
cDNA サイズ:660bp
cDNA の説明:Full length Clone DNA of Rattus norvegicus nudix (nucleoside diphosphate linked moiety X)-type motif 5 with C terminal Flag tag.
遺伝子の別名:Nudt5
:Rat
ベクター:pCMV3-C-FLAG
Plasmid:
制限サイト:
タグ シーケンス:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

ラット ADP-sugar Pyrophosphatase/NUDT5 Gene ORF cDNA clone expression plasmid, C-Flag タグ on other vectors
ラット ADP-sugar Pyrophosphatase/NUDT5 Gene ORF cDNA clone expression plasmid, C-GFPSpark タグRG81109-ACGJPY54410
ラット ADP-sugar Pyrophosphatase/NUDT5 Gene ORF cDNA clone expression plasmid, C-OFPSpark タグRG81109-ACRJPY54410
ラット ADP-sugar Pyrophosphatase/NUDT5 Gene ORF cDNA clone expression plasmid, N-GFPSpark タグRG81109-ANGJPY54410
ラット ADP-sugar Pyrophosphatase/NUDT5 Gene ORF cDNA clone expression plasmid, N-OFPSpark タグRG81109-ANRJPY54410
ラット ADP-sugar Pyrophosphatase/NUDT5 Gene ORF cDNA clone expression plasmid, C-Flag タグRG81109-CFJPY47150
ラット ADP-sugar Pyrophosphatase/NUDT5 Gene ORF cDNA clone expression plasmid, C-His タグRG81109-CHJPY47150
ラット ADP-sugar Pyrophosphatase/NUDT5 Gene ORF cDNA clone expression plasmid, C-Myc タグRG81109-CMJPY47150
ラット ADP-sugar Pyrophosphatase/NUDT5 Gene ORF cDNA clone expression plasmid, C-HA タグRG81109-CYJPY47150
ラット ADP-sugar Pyrophosphatase/NUDT5 Gene ORF cDNA clone expression plasmid, N-Flag タグRG81109-NFJPY47150
ラット ADP-sugar Pyrophosphatase/NUDT5 Gene ORF cDNA clone expression plasmid, N-His タグRG81109-NHJPY47150
ラット ADP-sugar Pyrophosphatase/NUDT5 Gene ORF cDNA clone expression plasmid, N-Myc タグRG81109-NMJPY47150
ラット ADP-sugar Pyrophosphatase/NUDT5 Gene ORF cDNA clone expression plasmid, N-HA タグRG81109-NYJPY47150
ラット ADP-sugar Pyrophosphatase/NUDT5 Gene ORF cDNA clone in cloning vectorRG81109-UJPY18140
ラット ADP-sugar Pyrophosphatase/NUDT5 Gene ORF cDNA clone expression plasmidRG81109-UTJPY47150
 発現ベクターの詳細情報
Product nameProduct name
背景

ADP-sugar Pyrophosphatase, also known as NUDT5, eliminates toxic nucleotide derivatives from the cell and regulate the levels of important signaling nucleotides and their metabolites. NUDT5 functions as a MutT-related protein and catalyzes the hydrolysis of 8-oxoGDP to 8-oxoGMP, thereby preventing misincorporation of 8-oxoGua into RNA. NUDT5 may play significant roles in regulating the G1-S transition in mammalian cells. It can also hydrolyze other nucleotide sugars with low activity.

参考文献
  • Ishibashi T. et al., 2004, EMBO Rep. 4 (5): 479-8.
  • Gerhard DS. et al., 2004, Genome Res. 14 (10B): 2121-7.
  • Rush J. et al., 2005, Nat Biotechnol. 23 (1): 94-101.
  • Kamiya H. et al., 2009, DNA Repair. 8 (10): 1250-4.
  • Size / Price
    カタログ: RG81109-CF
    定価: 
    価格:      (You Save: )
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