|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat IL21 ORF mammalian expression plasmid, C-GFPSpark tag||RG80200-ACG|
|Rat IL21 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80200-ACR|
|Rat IL21 ORF mammalian expression plasmid, C-Flag tag||RG80200-CF|
|Rat IL21 ORF mammalian expression plasmid, C-His tag||RG80200-CH|
|Rat IL21 ORF mammalian expression plasmid, C-Myc tag||RG80200-CM|
|Rat IL21 ORF mammalian expression plasmid, C-HA tag||RG80200-CY|
|Rat IL21 Gene cDNA clone plasmid||RG80200-G|
|Rat IL21 ORF mammalian expression plasmid, N-Flag tag||RG80200-NF|
|Rat IL21 ORF mammalian expression plasmid, N-His tag||RG80200-NH|
|Rat IL21 ORF mammalian expression plasmid, N-Myc tag||RG80200-NM|
|Rat IL21 ORF mammalian expression plasmid, N-HA tag||RG80200-NY|
|Rat IL21 natural ORF mammalian expression plasmid||RG80200-UT|
IL21 belongs to the IL-15/IL-21 family. It is a cytokine with immunoregulatory activity. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. IL21 is expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. It may promote the transition between innate and adaptive immunity. IL-21 has been tried as therapy for alleviating allergic responses. It can significantly decrease pro-inflammatory cytokines produced by T cells in addition to decreasing IgE levels in a mouse model for rhinitis (nasal passage inflammation)