|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|ラット IL18R1 Gene ORF cDNA clone expression plasmid, C-GFPSpark タグ||RG80191-ACG|
|ラット IL18R1 Gene ORF cDNA clone expression plasmid, C-OFPSpark タグ||RG80191-ACR|
|ラット IL18R1 Gene ORF cDNA clone expression plasmid, C-Flag タグ||RG80191-CF|
|ラット IL18R1 Gene ORF cDNA clone expression plasmid, C-His タグ||RG80191-CH|
|ラット IL18R1 Gene ORF cDNA clone expression plasmid, C-Myc タグ||RG80191-CM|
|ラット IL18R1 Gene ORF cDNA clone expression plasmid, C-HA タグ||RG80191-CY|
|ラット IL18R1 Gene ORF cDNA clone in cloning vector||RG80191-G|
|ラット IL18R1 Gene ORF cDNA clone expression plasmid, N-Flag タグ||RG80191-NF|
|ラット IL18R1 Gene ORF cDNA clone expression plasmid, N-His タグ||RG80191-NH|
|ラット IL18R1 Gene ORF cDNA clone expression plasmid, N-Myc タグ||RG80191-NM|
|ラット IL18R1 Gene ORF cDNA clone expression plasmid, N-HA タグ||RG80191-NY|
|ラット IL18R1 Gene ORF cDNA clone expression plasmid||RG80191-UT|
Interleukin-18 receptor 1 (IL18R1) also known as CD218 antigen-like family member A, CDw218a, IL1 receptor-related protein and CD218a, is an interleukin receptor of the immunoglobulin superfamily. IL18R1 is found expressed in lung, leukocytes, spleen, liver, thymus, prostate, small intestine, colon, placenta, and heart, and is absent from brain, skeletal muscle, pancreas, and kidney. High level of expression is found in Hodgkin disease cell lines. This receptor is specifically binds interleukin 18 (IL18), and is essential for IL18 mediated signal transduction. IL18R1 contains 3 Ig-like C2-type (immunoglobulin-like) domains and 1 TIR domain. It is a single-pass type I membrane protein. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells. The increased expression of IL18R1 may contribute pathogenically to disease and is therefore a potential therapeutic target. The absence of a genetic association in the IL18R1 gene itself suggests regulation from other parts of the genome, or as part of the inflammatory cascade in multiple sclerosis without a prime genetic cause.