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ラット Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, N-HA タグ

データシートレビュー関連製品プロトコル
Rat GOT1 cDNA クローン製品情報
Gene_bank_ref_id:NM_012571.2
cDNA サイズ:1242bp
cDNA の説明:Full length Clone DNA of Rattus norvegicus glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) with N terminal HA tag.
遺伝子の別名:cCAT, Aspat, Gaspat, cAspAT
:Rat
ベクター:pCMV3-N-HA
Plasmid:
制限サイト:
タグ シーケンス:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

ラット Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, N-HA タグ on other vectors
ラット Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, C-GFPSpark タグRG80493-ACGJPY54410
ラット Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, C-OFPSpark タグRG80493-ACRJPY54410
ラット Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, N-GFPSpark タグRG80493-ANGJPY54410
ラット Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, N-OFPSpark タグRG80493-ANRJPY54410
ラット Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, C-Flag タグRG80493-CFJPY47150
ラット Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, C-His タグRG80493-CHJPY47150
ラット Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, C-Myc タグRG80493-CMJPY47150
ラット Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, C-HA タグRG80493-CYJPY47150
ラット Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, N-Flag タグRG80493-NFJPY47150
ラット Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, N-His タグRG80493-NHJPY47150
ラット Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, N-Myc タグRG80493-NMJPY47150
ラット Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, N-HA タグRG80493-NYJPY47150
ラット Aspartate aminotransferase / GOT1 Gene ORF cDNA clone in cloning vectorRG80493-UJPY18140
ラット Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmidRG80493-UTJPY47150
 発現ベクターの詳細情報
Product nameProduct name
背景

Aspartate aminotransferase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, aspartate aminotransferase and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. There is a rare in-frame deletion in aspartate aminotransferase gene, which inactivates cytosolic aspartate aminotransferase(cAST) enzyme in the Old Order Amish. This may help to understand structure and function of the enzyme and would be useful for predicting serum aspartate AST levels.

参考文献
  • Shen H, et al. (2011) Genome-wide association study identifies genetic variants in GOT1 determining serum aspartate aminotransferase levels. J Hum Genet. 56(11):801-5.
  • Doonan S, et al. (1985) Structural and genetic relationships between cytosolic and mitochondrial isoenzymes. Int J Biochem. 16(12):1193-9.
  • Panteghini M. (1990) Aspartate aminotransferase isoenzymes. Clin Biochem. 23(4):311-9.
  • Bousquet-Lemercier B, et al. (1990) Properties of human liver cytosolic aspartate aminotransferase mRNAs generated by alternative polyadenylation site selection. Biochemistry. 29(22):5293-9.
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