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ラット glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmid, C-Myc タグ

    データシートレビュー関連製品プロトコル
    ラット GLRX cDNA クローン製品情報
    Gene_bank_ref_id:NM_022278.1
    cDNA サイズ:324bp
    cDNA の説明:Full length Clone DNA of Rattus norvegicus glutaredoxin (thioltransferase) with C terminal Myc tag.
    遺伝子の別名:Grx, Glrx1
    :Rat
    ベクター:pCMV3-C-Myc
    Plasmid:
    制限サイト:
    タグ シーケンス:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
    シーケンスの説明:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with GLRX qPCR primers for gene expression analysis, RP301058 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    保存:The lyophilized plasmid can be stored at room temperature for three months.
    Myc Tag Info

    A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

    A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

    The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

    ラット glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmid, C-Myc タグ on other vectors
    ラット glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmid, C-GFPSpark タグRG81101-ACGJPY54410
    ラット glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmid, C-OFPSpark タグRG81101-ACRJPY54410
    ラット glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmid, N-GFPSpark タグRG81101-ANGJPY54410
    ラット glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmid, N-OFPSpark タグRG81101-ANRJPY54410
    ラット glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmid, C-Flag タグRG81101-CFJPY47150
    ラット glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmid, C-His タグRG81101-CHJPY47150
    ラット glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmid, C-Myc タグRG81101-CMJPY47150
    ラット glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmid, C-HA タグRG81101-CYJPY47150
    ラット glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmid, N-Flag タグRG81101-NFJPY47150
    ラット glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmid, N-His タグRG81101-NHJPY47150
    ラット glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmid, N-Myc タグRG81101-NMJPY47150
    ラット glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmid, N-HA タグRG81101-NYJPY47150
    ラット glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone in cloning vectorRG81101-UJPY18140
    ラット glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmidRG81101-UTJPY47150
     発現ベクターの詳細情報
    Product nameProduct name
    背景

    Glutaredoxin-1, also known as GRX1 and GLRX, belongs to the glutaredoxin family. Glutaredoxins are small redox enzymes that use glutathione as a cofactor. Glutaredoxins are oxidized by substrates, and reduced non-enzymatically by glutathione. Glutaredoxin-1 functions as an electron carrier in the glutathione-dependent synthesis of deoxyribonucleotides by the enzyme ribonucleotide reductase. Glutaredoxin-1 exists in either a reduced or an oxidized form. Glutaredoxins function as electron carriers in the glutathione-dependent synthesis of deoxyribonucleotides by the enzymeribonucleotide reductase.

    参考文献
  • Holmgren A. et al., 1988, FEMS Microbiol Rev. 4 (4): 271-97.
  • Holmgren A. 1988, Biochem Soc Trans. 16 (2): 95-6.
  • Holmgren A. 1989, J Biol Chem. 264 (24): 13963-6.
  • Size / Price
    カタログ: RG81101-CM
    定価: 
    価格:      (You Save: )

    Datasheet & Documentation

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