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ラット FTL/ferritin, light polypeptide Gene ORF cDNA clone expression plasmid, N-HA タグ

データシートレビュー関連製品プロトコル
Rat FTL cDNA クローン製品情報
Gene_bank_ref_id:BC061525.1
cDNA サイズ:552bp
cDNA の説明:Full length Clone DNA of Rattus norvegicus ferritin, light polypeptide with N terminal HA tag.
遺伝子の別名:Ftl1
:Rat
ベクター:pCMV3-N-HA
Plasmid:
制限サイト:
タグ シーケンス:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

ラット FTL/ferritin, light polypeptide Gene ORF cDNA clone expression plasmid, N-HA タグ on other vectors
ラット FTL/ferritin, light polypeptide Gene ORF cDNA clone expression plasmid, C-GFPSpark タグRG80491-ACGJPY54410
ラット FTL/ferritin, light polypeptide Gene ORF cDNA clone expression plasmid, C-OFPSpark タグRG80491-ACRJPY54410
ラット FTL/ferritin, light polypeptide Gene ORF cDNA clone expression plasmid, N-GFPSpark タグRG80491-ANGJPY54410
ラット FTL/ferritin, light polypeptide Gene ORF cDNA clone expression plasmid, N-OFPSpark タグRG80491-ANRJPY54410
ラット FTL/ferritin, light polypeptide Gene ORF cDNA clone expression plasmid, C-Flag タグRG80491-CFJPY47150
ラット FTL/ferritin, light polypeptide Gene ORF cDNA clone expression plasmid, C-His タグRG80491-CHJPY47150
ラット FTL/ferritin, light polypeptide Gene ORF cDNA clone expression plasmid, C-Myc タグRG80491-CMJPY47150
ラット FTL/ferritin, light polypeptide Gene ORF cDNA clone expression plasmid, C-HA タグRG80491-CYJPY47150
ラット FTL/ferritin, light polypeptide Gene ORF cDNA clone expression plasmid, N-Flag タグRG80491-NFJPY47150
ラット FTL/ferritin, light polypeptide Gene ORF cDNA clone expression plasmid, N-His タグRG80491-NHJPY47150
ラット FTL/ferritin, light polypeptide Gene ORF cDNA clone expression plasmid, N-Myc タグRG80491-NMJPY47150
ラット FTL/ferritin, light polypeptide Gene ORF cDNA clone expression plasmid, N-HA タグRG80491-NYJPY47150
ラット FTL/ferritin, light polypeptide Gene ORF cDNA clone in cloning vectorRG80491-UJPY18140
ラット FTL/ferritin, light polypeptide Gene ORF cDNA clone expression plasmidRG80491-UTJPY47150
 発現ベクターの詳細情報
Product nameProduct name
背景

Ferritin, light polypeptide (FTL) is the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Storage of iron in the tissues occurs in the form of ferritin and hemosiderin. The latter originates from ferritin that has undergone intracellular digestion of its protein shell, leaving the iron core. Ferritin and hemosiderin are components of a continuum. Ferritin has been identified in all types of living organisms: animals, plants, molds, and bacteria. Whithin the protein shell of ferritin, iron is first oxidized to the ferric state for storage as ferric oxyhdroxide. Thus, ferritin removes excess iron from the cell sap where it could otherwise participate in peroxidation mechanisms.

参考文献
  • Munro HN, et al. (1988) The ferritin genes: structure, expression, and regulation. Ann N Y Acad Sci. 526: 113-23.
  • Zhang Y, et al. (2008) Comparative proteomic analysis of human placenta derived from assisted reproductive technology. Proteomics. 8 (20): 4344-56.
  • Lebo RV, et al. (1986) Human ferritin light chain gene sequences mapped to several sorted chromosomes. Hum Genet. 71 (4): 325-8.
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    カタログ: RG80491-NY
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