ENO3 qPCR Primer Pairs, Rat

Cat: RP300653
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ENO3 qPCR Primer Pairs, Rat General Information
Target Details
Species:
Rat
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
ENO3 Background Information

ENO3 is one of the three enolase isoenzymes found in mammals. As a homodimer, ENO3 is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in ENO3 gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for ENO3 gene that differ only in their 5' UTR. ENO3 may play a role in muscle development and regeneration. It appears to have a function in striated muscle development and regeneration.

Full Name
enolase 3 (beta, muscle)
References
  • Peshavaria M, et al. (1989) Structure of human muscle (beta) enolase mRNA and protein deduced from a genomic clone. Nucleic Acids Res. 17(21):8862.
  • Calì L, et al. (1990) Nucleotide sequence of a cDNA encoding the human muscle-specific enolase (MSE). Nucleic Acids Res. 18(7):1893.
  • Peshavaria M, et al. (1991) Molecular structure of the human muscle-specific enolase gene (ENO3). Biochem J. 275(2):427-33.
  • ENO3 qPCR Primer Pairs, Rat, Related Products

    Product Name Catalog#(PDF)
    ENO3 cDNA ORF Clone in Cloning Vector, Rat RG80689-U
    ENO3 cDNA ORF Clone, Rat, untagged RG80689-UT
    ENO3 cDNA ORF Clone, Rat, C-GFPSpark® tag RG80689-ACG
    ENO3 Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG80689-ACGLN
    ENO3 cDNA ORF Clone, Rat, N-GFPSpark® tag RG80689-ANG
    ENO3 cDNA ORF Clone, Rat, C-OFPSpark® tag RG80689-ACR
    ENO3 cDNA ORF Clone, Rat, N-OFPSpark® tag RG80689-ANR
    ENO3 cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80689-CF
    ENO3 cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80689-NF
    ENO3 cDNA ORF Clone, Rat, C-His tag RG80689-CH
    ENO3 cDNA ORF Clone, Rat, N-His tag RG80689-NH
    ENO3 cDNA ORF Clone, Rat, C-HA tag RG80689-CY
    ENO3 cDNA ORF Clone, Rat, N-HA tag RG80689-NY
    ENO3 cDNA ORF Clone, Rat, C-Myc tag RG80689-CM
    ENO3 cDNA ORF Clone, Rat, N-Myc tag RG80689-NM

    ENO3 qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    ENO3 cDNA ORF Clone in Cloning Vector, Mouse MG52862-G
    ENO3 cDNA ORF Clone, Mouse, untagged MG52862-UT
    ENO3 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG52862-ACG
    ENO3 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG52862-ACGLN
    ENO3 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG52862-ACR
    ENO3 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG52862-CF
    ENO3 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG52862-NF
    ENO3 cDNA ORF Clone, Mouse, C-His tag MG52862-CH
    ENO3 cDNA ORF Clone, Mouse, N-His tag MG52862-NH
    ENO3 cDNA ORF Clone, Mouse, C-HA tag MG52862-CY
    ENO3 cDNA ORF Clone, Mouse, N-HA tag MG52862-NY
    ENO3 cDNA ORF Clone, Mouse, C-Myc tag MG52862-CM
    ENO3 cDNA ORF Clone, Mouse, N-Myc tag MG52862-NM

    ENO3 qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    ENO3 cDNA ORF Clone in Cloning Vector, Human HG14270-G
    ENO3 cDNA ORF Clone, Human, untagged HG14270-UT
    ENO3 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG14270-ACGLN
    ENO3 cDNA ORF Clone, Human, C-GFPSpark® tag HG14270-ACG
    ENO3 cDNA ORF Clone, Human, N-GFPSpark® tag HG14270-ANG
    ENO3 cDNA ORF Clone, Human, C-OFPSpark® tag HG14270-ACR
    ENO3 cDNA ORF Clone, Human, N-OFPSpark® tag HG14270-ANR
    ENO3 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG14270-CF
    ENO3 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG14270-NF
    ENO3 cDNA ORF Clone, Human, C-His tag HG14270-CH
    ENO3 cDNA ORF Clone, Human, N-His tag HG14270-NH
    ENO3 cDNA ORF Clone, Human, C-HA tag HG14270-CY
    ENO3 cDNA ORF Clone, Human, N-HA tag HG14270-NY
    ENO3 cDNA ORF Clone, Human, C-Myc tag HG14270-CM
    ENO3 cDNA ORF Clone, Human, N-Myc tag HG14270-NM
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