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ラット ADSL / Adenylosuccinate Lyase Gene ORF cDNA clone expression plasmid, N-Flag タグ

    データシートレビュー関連製品プロトコル
    ラット ADSL cDNA クローン製品情報
    Gene_bank_ref_id:NM_001130503.1
    cDNA サイズ:1455bp
    cDNA の説明:Full length Clone DNA of Rattus norvegicus adenylosuccinate lyase with N terminal Flag tag.
    遺伝子の別名:null
    :Rat
    ベクター:pCMV3-N-FLAG
    Plasmid:
    制限サイト:
    タグ シーケンス:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
    シーケンスの説明:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with ADSL qPCR primers for gene expression analysis, RP301295 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    保存:The lyophilized plasmid can be stored at room temperature for three months.
    FLAG Tag Info

    FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

    A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

    The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

    ラット ADSL / Adenylosuccinate Lyase Gene ORF cDNA clone expression plasmid, N-Flag タグ on other vectors
    ラット ADSL / Adenylosuccinate Lyase Gene ORF cDNA clone expression plasmid, C-GFPSpark タグRG81324-ACGJPY54410
    ラット ADSL / Adenylosuccinate Lyase Gene ORF cDNA clone expression plasmid, C-OFPSpark タグRG81324-ACRJPY54410
    ラット ADSL / Adenylosuccinate Lyase Gene ORF cDNA clone expression plasmid, N-GFPSpark タグRG81324-ANGJPY54410
    ラット ADSL / Adenylosuccinate Lyase Gene ORF cDNA clone expression plasmid, N-OFPSpark タグRG81324-ANRJPY54410
    ラット ADSL / Adenylosuccinate Lyase Gene ORF cDNA clone expression plasmid, C-Flag タグRG81324-CFJPY47150
    ラット ADSL / Adenylosuccinate Lyase Gene ORF cDNA clone expression plasmid, C-His タグRG81324-CHJPY47150
    ラット ADSL / Adenylosuccinate Lyase Gene ORF cDNA clone expression plasmid, C-Myc タグRG81324-CMJPY47150
    ラット ADSL / Adenylosuccinate Lyase Gene ORF cDNA clone expression plasmid, C-HA タグRG81324-CYJPY47150
    ラット ADSL / Adenylosuccinate Lyase Gene ORF cDNA clone in cloning vectorRG81324-GJPY18140
    ラット ADSL / Adenylosuccinate Lyase Gene ORF cDNA clone expression plasmid, N-Flag タグRG81324-NFJPY47150
    ラット ADSL / Adenylosuccinate Lyase Gene ORF cDNA clone expression plasmid, N-His タグRG81324-NHJPY47150
    ラット ADSL / Adenylosuccinate Lyase Gene ORF cDNA clone expression plasmid, N-Myc タグRG81324-NMJPY47150
    ラット ADSL / Adenylosuccinate Lyase Gene ORF cDNA clone expression plasmid, N-HA タグRG81324-NYJPY47150
    ラット ADSL / Adenylosuccinate Lyase Gene ORF cDNA clone expression plasmidRG81324-UTJPY47150
     発現ベクターの詳細情報
    Product nameProduct name
    背景

    Adenylosuccinate lyase, also known as adenylosuccinase, ADSL or ASL, is an enzyme implicated in the reaction of adenylosuccinat converting to AMP and fumarate as part of the purine nucleotide cycle. The two substates of adenylosuccinate lyase (ADSL) are dephosphorylated derivatives of SAICA ribotide (SAICAR) and adenylosuccinate (S-AMP), which catalyzes an important reaction in the de novo pathway of purine biosynthesis. ADSL catalyzes two distinct reactions in the synthesis of purine nucleotides, both of which involve the _-elimination of fumarate to produce either aminoimidazole carboxamide ribotide from SAICAR or AMP from S-AMP. The Adenylosuccinate lyase deficiency is a rare autosomal recessive metabolic disorder characterized by the present of SAICA riboside and succinyladenosine (S-Ado). ADSL defect in different patients is often caused by different mutations to the enzyme.

    参考文献
  • Nassogne M, et al. (2000) Adenylosuccinase deficiency: an unusual cause of early-onset epilepsy associated with acquired microcephaly. Brain and development. 22 (6): 383-6.
  • Sivendran S, et al. (2004) Two novel mutant human adenylosuccinate lyases (ASLs) associated with autism and characterization of the equivalent mutant Bacillus subtilis ASL. J Biol Chem. 279 (51): 53789-97.
  • Lee TT, et al. (1999) His68 and His141 are critical contributors to the intersubunit catalytic site of adenylosuccinate lyase of Bacillus subtilis. Biochemistry. 38 (1): 22-32.
  • Size / Price
    カタログ: RG81324-NF
    定価: 
    価格:      (You Save: )

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