|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PRMT5 ORF mammalian expression plasmid, C-GFPSpark tag||HG11074-ACG|
|Human PRMT5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11074-ACR|
|Human PRMT5 ORF mammalian expression plasmid, N-GFPSpark tag||HG11074-ANG|
|Human PRMT5 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11074-ANR|
|Human PRMT5 ORF mammalian expression plasmid, C-Flag tag||HG11074-CF|
|Human PRMT5 ORF mammalian expression plasmid, C-His tag||HG11074-CH|
|Human PRMT5 ORF mammalian expression plasmid, C-Myc tag||HG11074-CM|
|Human PRMT5 ORF mammalian expression plasmid, C-HA tag||HG11074-CY|
|人 PRMT5 基因全长ORF克隆||HG11074-M|
|Human PRMT5 ORF mammalian expression plasmid, N-Flag tag||HG11074-NF|
|Human PRMT5 ORF mammalian expression plasmid, N-His tag||HG11074-NH|
|Human PRMT5 ORF mammalian expression plasmid, N-Myc tag||HG11074-NM|
|Human PRMT5 ORF mammalian expression plasmid, N-HA tag||HG11074-NY|
|Human PRMT5 natural ORF mammalian expression plasmid||HG11074-UT|
Methylation of arginine residues is a widespread post-translational modification of proteins catalyzed by a small family of PRMTs. The modification appears to regulate protein functions and interactions that affect gene regulation, signalling and subcellular localization of proteins and nucleic acids. Protein arginine methyltransferase 5 (PRMT5) is a member of the protein arginine N-methyltransferases (PRMT)family, and exists as at least homodimers and homotetramers, or homooligomers mediated by disulfide bonds and non-covalent association ubiquitously. PRMT5 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3), and thus plays a role in the assembly and biogenesis of snRNP core particles. PRMT5 methylates histone H2A and H4 'Arg-3' during germ cell development, as well as histone H3 'Arg-8', which may repress transcription. PRMT5 also methylates SUPT5H and regulates its transcriptional elongation properties. Additionally, it is also suggested that PRMT5 negatively regulates cyclin E1 promoter activity and cellular proliferation.