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PPM1A / PP2CA / PP2C-alpha 抗体, マウスモノクローナル抗体

  • PPM1A / PP2CA / PP2C-alpha Antibody, Mouse MAb, Immunofluorescence
  • Human PPM1A/PP2CA Flow Cytometry (FC) 14795
データシートレビュー関連製品プロトコル
人々 PPM1A 抗体製品情報
免疫原:Recombinant Human PPM1A / PP2C-alpha protein (Catalog#12984-H08E)
Clone ID:01
Ig タイプ:Mouse IgG2a
濃度:
エンドトキシン:
製剤:0.2 μm filtered solution in PBS with 5% trehalose
調製:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PPM1A / PP2C-alpha (rh PPM1A / PP2C-alpha; Catalog#12984-H08E; P35813-1; Met 1-Trp 382). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
人々 PPM1A Antibody IF Application Image
PPM1A / PP2CA / PP2C-alpha Antibody, Mouse MAb, Immunofluorescence
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Caption:
Immunofluorescence staining of Human PPM1A in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human PPM1A monoclonal antibody (15 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Other PPM1A Antibody Products
PPM1A/PP2CA 背景

Protein phosphatase 1A (PPM1A / PP2CA) is an enzyme belonging to the PP2C family of Ser / Thr protein phosphatases. Members of PP2C family are negative regulators of cell stress response pathways and the MAP kinases and MAP kinase kinases. It has also been demonstrated to inhibit the activation of p38 and JNK kinase cascades. PPM1A dephosphorylates and promotes nuclear export of TGFβ-activated Smad2/3. Ectopic expression of PPM1A abolishes TGFβ-induced antiproliferative and transcriptional responses, whereas depletion of PPM1A enhances TGFβ signaling in mammalian cells. It has been demonstrated that PPM1A / PP2CA, through dephosphorylation of Smad2/3, plays a critical role in terminating TGFβ signaling. Overexpression of PPM1A is reported to activate the expression of the tumor suppressor gene TP53 / p53, which leads to cell apoptosis.

人々 PPM1A/PP2CA 参考文献
  • Lin X, et al. (2006) PPM1A functions as a Smad phosphatase to terminate TGFbeta signaling. Cell. 125(5): 915-28.
  • Marc F, et al. (2003) Protein phosphatase 2C binds selectively to and dephosphorylates metabotropic glutamate receptor 3. Proc Natl Acad. 100 (26): 16006-11.
  • Mann DJ, et al. (1992) Mammalian protein serine/threonine phosphatase 2C: cDNA cloning and comparative analysis of amino acid sequences. Biochim Biophys Acta. 1130 (1): 100-4.
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