Anti-PHGDH Antibody

Cat: 13167-MM05
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Anti-PHGDH Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-PHGDH Antibody
Validated applications
ELISA
Species reactivity
Reacts with: Human
Specificity
Human PHGDH
No cross-reactivity in ELISA with E.coli cell lysate
Immunogen
Recombinant Human PGDH / PHGDH protein (Catalog#13167-H08E)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PGDH / PHGDH (rh PGDH / PHGDH; Catalog#13167-H08E; O43175; Met1-Phe533). The IgG fraction of the cell culture supernatant was purified by Protein A affinity.
Source
Monoclonal Mouse IgG2b Clone #05
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-PHGDH Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-PHGDH Antibody Alternative Names
Anti-3-PGDH Antibody;Anti-3PGDH Antibody;Anti-HEL-S-113 Antibody;Anti-NLS Antibody;Anti-NLS1 Antibody;Anti-PDG Antibody;Anti-PGAD Antibody;Anti-PGD Antibody;Anti-PGDH Antibody;Anti-PHGDHD Antibody;Anti-SERA Antibody
PHGDH Background Information

PHGDH is a member of the D-isomer specific 2-hydroxyacid dehydrogenase family. This new family consists of D-isomer-stereospecific enzymes. The conserved residues in this family appear to be the residues involved in the substrate binding and the catalytic reaction, and thus to be targets for site-directed mutagenesis. A number of NAD-dependent 2-hydroxyacid dehydrogenases which seem to be specific for the D-isomer of their substrate have been shown to be functionally and structurally related. PHGDH catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. Overexpression of PHGDH may cause certain breast cancers. Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency which is characterized by congenital microcephaly, psychomotor retardation, and seizures.

Full Name
phosphoglycerate dehydrogenase
References
  • Pind S, et al. (2002) V490M, a common mutation in 3-phosphoglycerate dehydrogenase deficiency, causes enzyme deficiency by decreasing the yield of mature enzyme. J Biol Chem. 277 (9): 7136-43.
  • Du H, et al. (2010) 3-Phosphoglycerate dehydrogenase expression is regulated by HOXA10 in murine endometrium and human endometrial cells. Reproduction. 139 (1): 237-45.
  • Possemato R, et al. (2011) Functional genomics reveal that the serine synthesis pathway is essential in breast cancer. Nature. 476 (7360): 346-50.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-PHGDH Antibody Mouse Monoclonal FCM,ICC/IF,IF 13167-MM01
    Anti-PHGDH Antibody Rabbit Polyclonal ELISA 13167-RP01
    Anti-PHGDH Antibody Rabbit Polyclonal WB,ELISA,IP 13167-T52
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