Anti-Oct4 Antibody

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Anti-Oct4 Antibody (Rabbit Polyclonal 抗体) の製品情報

製品名
Anti-Oct4 Antibody
検証済のアプリケーション
ICC/IF
交差反応
Reacts with: Human
特異性
Human Oct4
免疫原
A synthetic peptide corresponding to the C-terminus of the Human Oct4 / POU5F1
調製
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Oct4 / POU5F1, and purified by antigen affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Oct4 Antibody (Rabbit Polyclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
ICC-IF 1:300-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Oct4 Antibody (Rabbit Polyclonal 抗体) の画像

Immunofluorescence staining of OCT4 in HESS9 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human OCT4 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green)Positive staining was localized to nucleus.

Anti-Oct4 Antibody: 別名

Anti-42280 Antibody; Anti-42281 Antibody; Anti-OTF-3 Antibody; Anti-OTF3 Antibody; Anti-OTF4 Antibody

Oct4 背景情報

The POU5F1 gene, which encodes the POU domain, class 5, transcription factor 1 (also known as Oct-4), is expressed in embryonic stem cells where it regulates pluripotency and proliferation.The POU5F1 gene encodes one of the "core" transcription factors necessary to establish and maintain pluripotency in mammals. Its function depends on its precise level of expression, so its transcription has to be tightly regulated. POU5F1 is a key regulator of self-renewal and differentiation in embryonic stem cells and may be associated with initiation, promotion, and progression in cancer. POU5F1 is a transcription factor and master regulator of cell pluripotency with indispensable roles in early embryo development and cell lineage specification. The role of embryonic POU5F1 in blastocyst formation and cell lineage specification differs between mammalian species .
完全な名称
POU class 5 homeobox 1

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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