CD73 cDNA ORF Clone, Mouse, untagged

1/1

This product is not available, please contact order_us@sinobiological.com for relevant product if required.

CD73 cDNA ORF Clone, Mouse, untagged: 製品情報

遺伝子
Mouse
NCBI 参考シーケンス番号
遺伝子の長さ
1731 bp
シーケンスの特徴
Identical with the Gene Bank Ref. ID sequence except for two point mutations: 9 C/T and 12 G/T not causing the amino acid variation.
製品の特徴
Full length Clone DNA of Mouse 5' nucleotidase, ecto.
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
pCMV/hygro
制限部位
NheI + XhoI (5.5kb + 1.73kb)
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Ampicillin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

CD73 cDNA ORF ヌクレオチド配列およびアミノ酸配列に関する情報

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

CD73 cDNA ORF Clone, Mouse, untagged: 検証済の画像

CD73 cDNA ORF Clone, Mouse, untagged: 別名

2210401F01Rik cDNA ORF Clone, Mouse; AI447961 cDNA ORF Clone, Mouse; CD73 cDNA ORF Clone, Mouse; eNT cDNA ORF Clone, Mouse; NT cDNA ORF Clone, Mouse; Nt5 cDNA ORF Clone, Mouse

CD73 背景情報

5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein which belongs to the5'-nucleotidase family. CD73 is a glycosyl phosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signalling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1 and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purigenic receptors to induce cellular responses. CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.
完全な名称
5'-nucleotidase, ecto (CD73)
参考文献
  • Resta R. et al., 1997, Cell Signal. 9 (2): 131-9.
  • Yamashita Y. et al., 1998, Eur J Immunol. 28 (10): 2981-90.
  • Louis NA. et al., 2008, J Immunol. 180 (6): 4246-55.
  • Grünewald JK. et al., 2010, J Inflamm. 7 (1): 10.
カートに追加しました カートに追加しませんでした カートを更新しています。少々お待ちください