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マウス TMED4 / ERS25 Gene ORF cDNA clone in cloning vector

データシートレビュー関連製品プロトコル
Mouse TMED4 cDNA クローン製品情報
Gene_bank_ref_id:NM_134020.1
cDNA サイズ:684bp
cDNA の説明:Full length Clone DNA of Mus musculus transmembrane emp24 protein transport domain containing 4.
遺伝子の別名:AI326346, 1110014L17Rik
:Mouse
ベクター:PGEM-T Vector
Plasmid:pGEM-mTMED4
制限サイト:
タグ シーケンス:
シーケンスの説明:Identical with the Gene Bank Ref. ID sequence except for the point mutation: 309A>G not causing the amino acid variation.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
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背景

TMED4, also known as ERS25, belongs to the EMP24/GP25L family. TMED4 may play a role in the regulation of heat-shock response and apoptosis. It is involved in vesicular protein trafficking, mainly in the early secretory pathway. TMED4 may also play a role in the biosynthesis of secreted cargo including processing. It functions in the regulation of heat-shock response and apoptosis. TMED4 also is involved in endoplasmic reticulum stress response.

参考文献
  • Hartley JL. et al., 2001, Genome Res. 10 (11): 1788-95.
  • Matoba R. et al., 1994, Gene. 146 (2): 199-207.
  • Matsuda A. et al., 2003, Oncogene. 22 (21): 3307-18.
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    カタログ: MG52950-G
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