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マウス Serpinb6b Gene ORF cDNA clone expression plasmid, C-OFPSpark タグ

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    マウス SERPINB6B cDNA クローン製品情報
    Gene_bank_ref_id:NM_011454.1
    cDNA サイズ:1134bp
    cDNA の説明:Full length Clone DNA of Mus musculus serine (or cysteine) peptidase inhibitor, clade B, member 6b with C terminal OFPSpark / RFP tag.
    遺伝子の別名:NK13, Spi12, ovalbumin, Serpinb6b
    :Mouse
    ベクター:pCMV3-C-OFPSpark
    Plasmid:
    制限サイト:
    タグ シーケンス:OFPSpark Tag: GATAGCACTGAG……CACCTGTTCCAG
    シーケンスの説明:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with Serpinb6b qPCR primers for gene expression analysis, MP200391 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    保存:The lyophilized plasmid can be stored at room temperature for three months.
    OFP (OFPSpark) / RFP Tag Info

    OFPSpark is a red (orange) fluorescent protein (excitation/emission maxima are 549 and 566 nm, respectively) derived from DsRed. Possessing high photostability and pH stability, OFPSpark is more than twice brighter than mOrange2. Fast OFPSpark maturation makes it clearly detectable in mammalian cells as early as within 8 hrs after transfection. OFPSpark can be expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with OFPSpark expression vectors produce bright fluorescence in 8 hrs after transfection. No cytotoxic effects or visible protein aggregation are observed. For its monomer structure, OFPSpark performs well in some fusions and protein labeling applications.

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    参考文献
  • Morgenstern KA, et al. (1994) Complementary DNA cloning and kinetic characterization of a novel intracellular serine proteinase inhibitor: mechanism of action with trypsin and factor Xa as model proteinases. Biochemistry. 33(11): 3432-41.
  • Strik MC, et al. (2004) Intracellular serpin SERPINB6 (PI6) is abundantly expressed by human mast cells and forms complexes with beta-tryptase monomers. Blood.103(7): 2710-7.
  • Scott FL, et al. (2007) SerpinB6 is an inhibitor of kallikrein-8 in keratinocytes. J Biochem. 142(4): 435-42.
  • Size / Price
    カタログ: MG50371-ACR
    定価: 
    価格:      (You Save: )

    Datasheet & Documentation

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