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マウス Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Myc タグ

データシートレビュー関連製品プロトコル
Mouse SEMA4A cDNA クローン製品情報
Gene_bank_ref_id:NM_013658.3
cDNA サイズ:2283bp
cDNA の説明:Full length Clone DNA of Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A, transcript variant 1 with C terminal Myc tag.
遺伝子の別名:SemB, Semab, AI132332, Sema4a
:Mouse
ベクター:pCMV3-C-Myc
Plasmid:
制限サイト:
タグ シーケンス:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

マウス Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Myc タグ on other vectors
マウス Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 Gene ORF cDNA clone expression plasmid, C-GFPSpark タグMG50330-ACGJPY59240
マウス Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 Gene ORF cDNA clone expression plasmid, C-OFPSpark タグMG50330-ACRJPY59240
マウス Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag タグMG50330-CFJPY51990
マウス Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 Gene ORF cDNA clone expression plasmid, C-His タグMG50330-CHJPY51990
マウス Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Myc タグMG50330-CMJPY51990
マウス Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 Gene ORF cDNA clone expression plasmid, C-HA タグMG50330-CYJPY51990
マウス Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 Gene ORF cDNA clone in cloning vectorMG50330-MJPY18140
マウス Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Flag タグMG50330-NFJPY51990
マウス Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 Gene ORF cDNA clone expression plasmid, N-His タグMG50330-NHJPY51990
マウス Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Myc タグMG50330-NMJPY51990
マウス Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 Gene ORF cDNA clone expression plasmid, N-HA タグMG50330-NYJPY51990
マウス Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 Gene ORF cDNA clone expression plasmidMG50330-UTJPY51990
 発現ベクターの詳細情報
Product nameProduct name
背景

Semaphorin-4A, also known as Semaphorin-B, SEMA4A, Sema B and SEMAB, is a single-pass type I  membrane protein which belongs to the semaphorin family. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. Semaphorin-4A / SEMA4A contains one Ig-like C2-type (immunoglobulin-like) domain, one PSI domain and one Sema domain. Defects in SEMA4A are the cause of retinitis pigmentosa type 35 (RP35) which leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Defects in SEMA4A are also the cause of cone-rod dystrophy type 10 (CORD10) which are inherited retinal dystrophies belonging to the group of pigmentary retinopathies. CORDs are characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration.

Semaphorins are secreted, transmembrane, and GPI-linked proteins, defined by cysteine-rich semaphorin protein domains, that have important roles in a variety of tissues. Humans have 20 semaphorins, Drosophila has five, and two are known from DNA viruses. Semaphorins are found in nematodes and crustaceans but not in non-animals. They are grouped into eight classes on the basis of phylogenetic tree analyses and the presence of additional protein motifs. Semaphorins have been implicated in diverse developmental processes such as axon guidance during nervous system development and regulation of cell migration.

参考文献
  • Clark H.F., et al., 2003, Genome Res. 13: 2265-2270.
  • Ota T., et al., 2004,Nat. Genet. 36: 40-45.
  • Neufeld, G. et al., 2005, Front Biosci. 10 : 751-60.
  • Fiore,R. et al., 2005, Mol Cell Biol. 25 (6):2310-9.
  • Abid A., et al., 2006, J. Med. Genet. 43:378-381.
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    カタログ: MG50330-CM
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