|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|マウス ROBO4 Gene ORF cDNA clone expression plasmid, C-GFPSpark タグ||MG51081-ACG|
|マウス ROBO4 Gene ORF cDNA clone expression plasmid, C-OFPSpark タグ||MG51081-ACR|
|マウス ROBO4 Gene ORF cDNA clone expression plasmid, C-Flag タグ||MG51081-CF|
|マウス ROBO4 Gene ORF cDNA clone expression plasmid, C-His タグ||MG51081-CH|
|マウス ROBO4 Gene ORF cDNA clone expression plasmid, C-Myc タグ||MG51081-CM|
|マウス ROBO4 Gene ORF cDNA clone expression plasmid, C-HA タグ||MG51081-CY|
|マウス ROBO4 Gene ORF cDNA clone in cloning vector||MG51081-G|
|マウス ROBO4 Gene ORF cDNA clone expression plasmid, N-Flag タグ||MG51081-NF|
|マウス ROBO4 Gene ORF cDNA clone expression plasmid, N-His タグ||MG51081-NH|
|マウス ROBO4 Gene ORF cDNA clone expression plasmid, N-Myc タグ||MG51081-NM|
|マウス ROBO4 Gene ORF cDNA clone expression plasmid, N-HA タグ||MG51081-NY|
|マウス ROBO4 Gene ORF cDNA clone expression plasmid||MG51081-UT|
Roundabout homolog 4, also known as magic roundabout and ROBO4 is a member of the immunoglobulin superfamily and ROBO family. ROBO4 is specifically expressed in endothelial cells. It is expressed at sites of angiogenesis in different tumor types. ROBO4 contains two fibronectin type-III domains and two Ig-like C2-type (immunoglobulin-like) domains. ROBO4 is the fourth identified member of the roundabout receptor family. It is the only Robo family member expressed in primary endothelial cells and that application of Slit inhibits their migration. ROBO4 is predominantly expressed in embryonic or tumor vascular endothelium and is considered important for vascular development and as a candidate tumor endothelial marker. ROBO4 is a bona fide member of the Robo family and may provide a repulsive cue to migrating endothelial cells during vascular development. ROBO4 is a receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. ROBO4 may mediate the inhibition of primary endothelial cell migration by Slit proteins. Activating ROBO4 may have broad therapeutic application in diseases characterized by excessive angiogenesis and/or vascular leak.