|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|マウス PDGF-C Gene ORF cDNA clone expression plasmid, C-GFPSpark タグ||MG50458-ACG|
|マウス PDGF-C Gene ORF cDNA clone expression plasmid, C-OFPSpark タグ||MG50458-ACR|
|マウス PDGF-C Gene ORF cDNA clone expression plasmid, C-Flag タグ||MG50458-CF|
|マウス PDGF-C Gene ORF cDNA clone expression plasmid, C-His タグ||MG50458-CH|
|マウス PDGF-C Gene ORF cDNA clone expression plasmid, C-Myc タグ||MG50458-CM|
|マウス PDGF-C Gene ORF cDNA clone expression plasmid, C-HA タグ||MG50458-CY|
|マウス PDGF-C Gene ORF cDNA clone in cloning vector||MG50458-M|
|マウス PDGF-C Gene ORF cDNA clone expression plasmid, N-Flag タグ||MG50458-NF|
|マウス PDGF-C Gene ORF cDNA clone expression plasmid, N-His タグ||MG50458-NH|
|マウス PDGF-C Gene ORF cDNA clone expression plasmid, N-Myc タグ||MG50458-NM|
|マウス PDGF-C Gene ORF cDNA clone expression plasmid, N-HA タグ||MG50458-NY|
|マウス PDGF-C Gene ORF cDNA clone expression plasmid||MG50458-UT|
PDGF-C is a member of the PDGF/VEGF family of growth factors with a unique domain organization and expression pattern. Platelet-derived growth factor receptors (PDGFRs) are catalytic receptors that have intracellular tyrosine kinase activity. They have roles in the regulation of many biological processes including embryonic development, angiogenesis, cell proliferation and differentiation, and contribute to the pathophysiology of some diseases, including cancer. There are two isoforms of the PDGFR receptor; PDGFRalpha and PDGFRbeta, which can form homo- or heterodimers. The endogenous PDGFR ligands are PDGF-A, -B, -C and -D, which induce receptor dimerization and transphosphorylation at specific tyrosine residues upon binding. This activates the intracellular kinase activity, initiating intracellular signaling through the MAPK, PI 3-K and PKCgamma pathways. PDGF-C acts as a specific ligand for alpha platelet-derived growth factor receptor homodimer, and alpha and beta heterodimer. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. PDGF-C Appears to be involved in the three stages of wound healing: inflammation, proliferation and remodeling. Involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs.