( We provide with MS4A1 qPCR primers for gene expression analysis, MP200417 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|マウス CD20/MS4A1 Gene ORF cDNA clone expression plasmid, C-GFPSpark タグ||MG50399-ACG|
|マウス CD20/MS4A1 Gene ORF cDNA clone expression plasmid, C-OFPSpark タグ||MG50399-ACR|
|マウス CD20/MS4A1 Gene ORF cDNA clone expression plasmid, C-Flag タグ||MG50399-CF|
|マウス CD20/MS4A1 Gene ORF cDNA clone expression plasmid, C-His タグ||MG50399-CH|
|マウス CD20/MS4A1 Gene ORF cDNA clone expression plasmid, C-Myc タグ||MG50399-CM|
|マウス CD20/MS4A1 Gene ORF cDNA clone expression plasmid, C-HA タグ||MG50399-CY|
|マウス CD20/MS4A1 Gene ORF cDNA clone in cloning vector||MG50399-G|
|マウス CD20/MS4A1 Gene ORF cDNA clone expression plasmid||MG50399-G-N|
|マウス CD20/MS4A1 Gene ORF cDNA clone expression plasmid, N-Flag タグ||MG50399-NF|
|マウス CD20/MS4A1 Gene ORF cDNA clone expression plasmid, N-His タグ||MG50399-NH|
|マウス CD20/MS4A1 Gene ORF cDNA clone expression plasmid, N-Myc タグ||MG50399-NM|
|マウス CD20/MS4A1 Gene ORF cDNA clone expression plasmid, N-HA タグ||MG50399-NY|
|マウス CD20/MS4A1 Gene ORF cDNA clone expression plasmid||MG50399-UT|
CD20 (membrane-spanning 4-domains, subfamily A, member 1), also known as MS4A1, is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. CD20 / MS4A1 is expressed on all stages of B cell development except the first and last. CD20 / MS4A1 is present from pre-pre B cells through memory cells, but not on either pro-B cells or plasma cells. It is a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. CD20 / MS4A1may be involved in the regulation of B-cell activation and proliferation. Defects in CD20 / MS4A1 are the cause of immunodeficiency common variable type 5(CVID5). CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low.